IRAP Antibody (E-12): sc-365051

IRAP Antibody (E-12) is a mouse monoclonal IgG3 antibody that detects IRAP (Insulin-responsive aminopeptidase) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). IRAP is a 1,025 amino acid protein predominantly expressed in the placenta, heart, kidney, and small intestine, with lower expression levels found in neuronal cells of the brain, skeletal muscle, spleen, liver, testis, and colon. IRAP is a member of the peptidase M1 family and plays a crucial role in the degradation of important hormones, including oxytocin, vasopressin, and angiotensin III, which are vital for maintaining homeostasis during pregnancy and regulating various physiological processes. IRAP is involved in the trafficking of the insulin-responsive glucose transporter Glut4, thereby influencing glucose uptake in cells, which is essential for metabolic regulation. IRAP interacts with Tankyrase-1 and Tankyrase-2, which are significant signaling targets of extracellular signal-regulated kinase (ERK) in the Golgi apparatus, highlighting IRAP′s role in cellular signaling pathways. The presence of three isoforms due to alternative splicing further underscores the complexity and functional diversity of IRAP, making anti-IRAP antibody (E-12) an invaluable tool for researchers studying IRAP′s multifaceted roles in health and disease.

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IRAP Antibody (E-12) References:

1. IRAP deficiency attenuates diet-induced obesity in mice through increased energy expenditure.  |  Niwa, M., et al. 2015. Biochem Biophys Res Commun. 457: 12-8. PMID: 25534853
2. 27-Hydroxycholesterol impairs neuronal glucose uptake through an IRAP/GLUT4 system dysregulation.  |  Ismail, MA., et al. 2017. J Exp Med. 214: 699-717. PMID: 28213512
3. IRAP+ endosomes restrict TLR9 activation and signaling.  |  Babdor, J., et al. 2017. Nat Immunol. 18: 509-518. PMID: 28319098
4. IRAP-dependent endosomal T cell receptor signalling is essential for T cell responses.  |  Evnouchidou, I., et al. 2020. Nat Commun. 11: 2779. PMID: 32487999
5. Enhanced recombinant expression and purification of human IRAP for biochemical and crystallography studies.  |  Sui, L. and Guo, HC. 2021. Biochem Biophys Rep. 27: 101042. PMID: 34169156
6. A Short ERAP2 That Binds IRAP Is Expressed in Macrophages Independently of Gene Variation.  |  Mattorre, B., et al. 2022. Int J Mol Sci. 23: PMID: 35563348
7. Development of a sandwich ELISA to detect circulating, soluble IRAP as a potential disease biomarker.  |  Vear, A., et al. 2023. Sci Rep. 13: 17565. PMID: 38001104
8. Old and New Biomarkers in Idiopathic Recurrent Acute Pericarditis (IRAP): Prognosis and Outcomes.  |  Mascolo, R., et al. 2025. Curr Cardiol Rep. 27: 17. PMID: 39798014
9. IRAP Drives Ribosomal Degradation to Refuel Energy for Platelet Activation during Septic Thrombosis.  |  Xu, B., et al. 2025. Adv Sci (Weinh). e2411914. PMID: 39853919
10. Discovery of New Nanomolar Selective IRAP Inhibitors.  |  He, B., et al. 2025. J Med Chem. 68: 4168-4195. PMID: 39916550

The preferred IRAP antibody is IRAP Antibody (F-5): sc-365300.