involucrin Antibody (SY5): sc-21748

Involucrin Antibody (SY5) is a mouse monoclonal IgG1 antibody that detects involucrin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-involucrin antibody (SY5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Involucrin plays a crucial role as a precursor protein in the formation of the keratinocyte cornified envelope, which is essential for skin barrier function and protection against environmental stressors. Involucrin is initially located in the cytosol of keratinocytes and becomes cross-linked to membrane proteins during terminal differentiation, a process vital for the structural integrity of the skin. The cross-linking occurs through covalent bonds formed between glutamine residues of involucrin and other envelope precursors, facilitated by transglutaminase enzymes. The extensive cross-linking ability of involucrin creates a robust and resilient cornified envelope, which is critical for maintaining skin hydration and preventing pathogen entry. Involucrin is specifically expressed in various cell types, including Chinese hamster ovarian cells, PtK2 rat kangaroo kidney cells, and rat epidermal keratinocytes, highlighting its importance in epithelial tissue development and function.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

involucrin Antibody (SY5) References:

1. Regulation of involucrin gene expression.  |  Eckert, RL., et al. 2004. J Invest Dermatol. 123: 13-22. PMID: 15191537
2. Biophysical characterization of involucrin reveals a molecule ideally suited to function as an intermolecular cross-bridge of the keratinocyte cornified envelope.  |  Yaffe, MB., et al. 1992. J Biol Chem. 267: 12233-8. PMID: 1601889
3. Stable expression of transfected human involucrin gene in various cell types: evidence for in situ cross-linking by type I and type II transglutaminase.  |  Rorke, EA. and Eckert, RL. 1991. J Invest Dermatol. 97: 543-8. PMID: 1678767
4. Structure and evolution of the human involucrin gene.  |  Eckert, RL. and Green, H. 1986. Cell. 46: 583-9. PMID: 2873896
5. Regulation of Filaggrin, Loricrin, and Involucrin by IL-4, IL-13, IL-17A, IL-22, AHR, and NRF2: Pathogenic Implications in Atopic Dermatitis.  |  Furue, M. 2020. Int J Mol Sci. 21: PMID: 32751111
6. Involucrin Modulates Vitamin D Receptor Activity in the Epidermis.  |  Schmidt, AD., et al. 2023. J Invest Dermatol. 143: 1052-1061.e3. PMID: 36642403
7. Brk/PTK6 and Involucrin Expression May Predict Breast Cancer Cell Responses to Vitamin D3.  |  Box, C., et al. 2023. Int J Mol Sci. 24: PMID: 37445934
8. Immunohistochemical evaluation of keratins and involucrin in differentiating between palmoplantar pustulosis and pompholyx.  |  Oh, DY., et al. 2024. Int J Dermatol. 63: 780-786. PMID: 38214207
9. Involucrin and other markers of keratinocyte terminal differentiation.  |  Watt, FM. 1983. J Invest Dermatol. 81: 100s-3s. PMID: 6345687
10. Tissue-specific and differentiation-appropriate expression of the human involucrin gene in transgenic mice: an abnormal epidermal phenotype.  |  Crish, JF., et al. 1993. Differentiation. 53: 191-200. PMID: 8405770
11. Psoriasis, involucrin, and protein kinase C.  |  Iizuka, H. and Takahashi, H. 1993. Int J Dermatol. 32: 333-8. PMID: 8505157
12. Repression of involucrin gene expression by transcriptional enhancer factor 1 (TEF-1).  |  Takahashi, H., et al. 1995. Arch Dermatol Res. 287: 740-6. PMID: 8554386

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-involucrin Antibody (SY5) has 67 citations in a variety of scientific publications.

Primary antibodies like Anti-involucrin Antibody (SY5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

involucrin Antibody (SY5) Specifications

Alternate Product Name: involucrin

Product Clone Name: SY5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids:

Epitope Target: involucrin

Epitope Species: human

Product Reactivity/Detection: involucrin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: porcine and canine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 67 citations in various scientific publications

Available Conjugates/Forms for involucrin Antibody (SY5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin