Intersectin Antibody (29): sc-136242

Intersectin Antibody (29) is a mouse monoclonal IgG1 kappa light chain antibody that detects the Intersectin protein of mouse, rat, and human origin by western blotting (WB) and immunoprecipitation (IP). Anti-Intersectin antibody (29) is available as a non-conjugated format. Intersectin, also known as Ese1 or ITSN1, plays a crucial role in cellular endocytic machinery, which is essential for membrane protein internalization and signaling pathway regulation. Intersectin (29) antibody recognizes a protein characterized by two N-terminal Ese15 homology (EH) domains, a central highly charged region, and five C-terminal SH3 domains. This structural arrangement enables Intersectin′s localization to Clathrin-coated pits at the plasma membrane through EH domains, while SH3 domains facilitate interactions with other endocytosis-related proteins, such as Dynamin. The central domain binds to SNAP 25, underscoring Intersectin′s importance in synaptic transmission and vesicle trafficking. Intersectin exists in two isoforms: Intersectin-short and Intersectin-long, produced through alternative splicing, with the long form specifically expressed in neurons and the short form found in glial and non-neuronal cells. Related proteins Intersectin-2 and murine homolog Ese2 share similar structural features and participate in endocytic scaffolding complex formation, highlighting Intersectin′s significance in maintaining cellular homeostasis and communication.

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Intersectin Antibody (29) References:

1. The EH and SH3 domain Ese proteins regulate endocytosis by linking to dynamin and Eps15.  |  Sengar, AS., et al. 1999. EMBO J. 18: 1159-71. PMID: 10064583
2. SH3-domain-containing proteins function at distinct steps in clathrin-coated vesicle formation.  |  Simpson, F., et al. 1999. Nat Cell Biol. 1: 119-24. PMID: 10559884
3. The endocytic protein intersectin is a major binding partner for the Ras exchange factor mSos1 in rat brain.  |  Tong, XK., et al. 2000. EMBO J. 19: 1263-71. PMID: 10716926

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Intersectin Antibody (29) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-Intersectin Antibody (29) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Intersectin Antibody (29) Specifications

Alternate Product Name: Intersectin

Product Clone Name: 29

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 800-909

Epitope Target: Intersectin

Epitope Species: mouse

Product Reactivity/Detection: Intersectin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for Intersectin Antibody (29): N/A