ING2 Antibody (B-5): sc-271544

ING2 Antibody (B-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects ING2 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ING2 (B-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ING2, also known as Inhibitor of Growth Protein 2, is a 280 amino acid protein encoded by the human gene ING2 and is a member of the ING family, characterized by the presence of a PHD-type zinc finger. ING2 plays a crucial role in regulating cell growth and apoptosis, particularly through involvement in the activation of the tumor suppressor p53/TP53 and the associated apoptotic pathways. ING2′s ability to enhance p53/TP53 acetylation is vital for function, as this promotes p53/TP53 transcriptional activity, essential for cellular responses to stress and DNA damage. ING2 is predominantly localized in the nucleus, where ING2 associates with chromatin and the nuclear matrix, and ING2 activity is modulated by binding to phosphoinositides, which can influence localization and function. ING2 expression is significantly elevated in colon cancer tissues compared to normal colon tissues, and ING2 can be induced by DNA-damaging agents such as etoposide and neocarzinostatin, highlighting potential roles in cancer biology and therapeutic responses.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

ING2 Antibody (B-5) References:

1. Cloning of a novel gene (ING1L) homologous to ING1, a candidate tumor suppressor.  |  Shimada, Y., et al. 1998. Cytogenet Cell Genet. 83: 232-5. PMID: 10072587
2. DNA damage-inducible gene p33ING2 negatively regulates cell proliferation through acetylation of p53.  |  Nagashima, M., et al. 2001. Proc Natl Acad Sci U S A. 98: 9671-6. PMID: 11481424
3. Role of the Sin3-histone deacetylase complex in growth regulation by the candidate tumor suppressor p33(ING1).  |  Kuzmichev, A., et al. 2002. Mol Cell Biol. 22: 835-48. PMID: 11784859
4. A novel PHD-finger motif protein, p47ING3, modulates p53-mediated transcription, cell cycle control, and apoptosis.  |  Nagashima, M., et al. 2003. Oncogene. 22: 343-50. PMID: 12545155
5. The PHD finger of the chromatin-associated protein ING2 functions as a nuclear phosphoinositide receptor.  |  Gozani, O., et al. 2003. Cell. 114: 99-111. PMID: 12859901
6. Loss of heterozygosity on chromosome 4q32-35 in sporadic basal cell carcinomas: evidence for the involvement of p33ING2/ING1L and SAP30 genes.  |  Sironi, E., et al. 2004. J Cutan Pathol. 31: 318-22. PMID: 15005689
7. Alterations in novel candidate tumor suppressor genes, ING1 and ING2 in human lung cancer.  |  Okano, T., et al. 2006. Oncol Rep. 15: 545-9. PMID: 16465410
8. The tumor suppressors p33ING1 and p33ING2 interact with alien in vivo and enhance alien-mediated gene silencing.  |  Fegers, I., et al. 2007. J Proteome Res. 6: 4182-8. PMID: 17929852

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ING2 Antibody (B-5) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-ING2 Antibody (B-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ING2 Antibody (B-5) Specifications

Alternate Product Name: ING2

Product Clone Name: B-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 111-181

Epitope Target: ING2

Epitope Species: human

Product Reactivity/Detection: inhibitor of growth protein 2

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for ING2 Antibody (B-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin