Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Importin-7 CRISPR/Cas9 KO Plasmid (h): sc-403562
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Importin-7 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Importin-7 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Importin-7 Antibody (E-2): sc-365231
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Importin-7 CRISPR/Cas9 KO Plasmid (h) | sc-403562 | 20 µg | $397.00 |
Overview
IPO7 encodes Importin-7, a karyopherin-β family nuclear transport receptor that mediates RanGTP-dependent import of diverse cargo proteins and ribonucleoprotein complexes through the nuclear pore. Importin-7 supports nuclear localization of regulatory factors involved in transcriptional control, ribosome biogenesis, and innate immune signaling, linking nucleocytoplasmic trafficking to cell-cycle progression and stress responses. Through its role in controlling nuclear availability of signaling and chromatin-associated proteins, IPO7 can influence pathways that govern proliferation and genome maintenance. Dysregulated nuclear transport has been associated with oncogenic programs and altered host–pathogen interactions, making IPO7 a useful node for mechanistic studies of transport-dependent phenotypes.
Importin-7 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the IPO7 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the IPO7 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the IPO7 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Importin-7 protein expression.
This CRISPR knockout system enables efficient generation of IPO7-deficient cell models for investigation of Importin-7 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting IPO7 exon(s) critical for Importin-7 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple IPO7 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Importin-7 CRISPR/Cas9 KO Plasmid (h) and Importin-7 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the IPO7 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Importin-7 HDR Plasmid (h) and Importin-7 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by IPO7 homology arms to support homology-directed repair at defined IPO7 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.