IL-1RI Antibody (H-8): sc-393998

IL-1RI Antibody (H-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects IL-1RI protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). IL-1RI (H-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. IL-1RI protein plays a crucial role in mediating the effects of interleukin-1, a key cytokine involved in inflammatory responses. IL-1RI is primarily located on the cell surface, where IL-1RI interacts with IL-1α and IL-1β ligands to initiate signaling pathways that lead to immune response activation, fever, and acute phase response. Proper functioning of IL-1RI is vital for maintaining homeostasis during inflammation, and dysregulation of IL-1RI can contribute to various inflammatory diseases. Additionally, IL-1 receptor antagonist, IL-1Rα, binds to IL-1RI without activation, serving as a natural inhibitor of IL-1 signaling. Understanding IL-1RI dynamics and ligand interactions is essential for developing therapeutic strategies targeting inflammatory conditions.

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IL-1RI Antibody (H-8) References:

1. The interleukin-1 system: receptors, ligands and signals.  |  Dower, SK., et al. 1992. Chem Immunol. 51: 33-64. PMID: 1533129
2. A novel IL-1 receptor, cloned from B cells by mammalian expression, is expressed in many cell types.  |  McMahan, CJ., et al. 1991. EMBO J. 10: 2821-32. PMID: 1833184
3. IL-1RI participates in normal growth plate development and bone modeling.  |  Simsa-Maziel, S., et al. 2013. Am J Physiol Endocrinol Metab. 305: E15-21. PMID: 23592480
4. Cloning the interleukin 1 receptor from human T cells.  |  Sims, JE., et al. 1989. Proc Natl Acad Sci U S A. 86: 8946-50. PMID: 2530587
5. MARCH3 attenuates IL-1β-triggered inflammation by mediating K48-linked polyubiquitination and degradation of IL-1RI.  |  Lin, H., et al. 2018. Proc Natl Acad Sci U S A. 115: 12483-12488. PMID: 30442668
6. High Expression of IL-1RI and EP₂ Receptors in the IL-1β/COX-2 Pathway, and a New Alternative to Non-Steroidal Drugs-Osthole in Inhibition COX-2.  |  Kordulewska, NK., et al. 2019. Int J Mol Sci. 20: PMID: 30620999
7. IL-4/IFN-γ inflammatory cytokine profile induces a deficient regulation of the IL-1β/IL-1RI/EP2/COX-2 pathway in nasal mucosa.  |  Machado-Carvalho, L., et al. 2019. Respir Med. 150: 136-140. PMID: 30961940
8. GAG positioning on IL-1RI; A mechanism regulated by dual effect of glycosylation.  |  Azimzadeh Irani, M. and Ejtehadi, MR. 2019. Glycobiology. 29: 803-812. PMID: 31317192
9. Binding of IL-1 alpha, IL-1 beta, and IL-1 receptor antagonist by soluble IL-1 receptors and levels of soluble IL-1 receptors in synovial fluids.  |  Arend, WP., et al. 1994. J Immunol. 153: 4766-74. PMID: 7963543
10. Elevated levels of shed type II IL-1 receptor in sepsis. Potential role for type II receptor in regulation of IL-1 responses.  |  Giri, JG., et al. 1994. J Immunol. 153: 5802-9. PMID: 7989776
11. Interleukin 1 signaling occurs exclusively via the type I receptor.  |  Sims, JE., et al. 1993. Proc Natl Acad Sci U S A. 90: 6155-9. PMID: 8327496
12. Independent binding of interleukin-1 alpha and interleukin-1 beta to type I and type II interleukin-1 receptors.  |  Slack, J., et al. 1993. J Biol Chem. 268: 2513-24. PMID: 8428929

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-IL-1RI Antibody (H-8) has 34 citations in a variety of scientific publications.

Primary antibodies like Anti-IL-1RI Antibody (H-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

IL-1RI Antibody (H-8) Specifications

Alternate Product Name: Interleukin 1 receptor type 1

Product Clone Name: H-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 539-563

Epitope Target: IL-1RI

Epitope Species: mouse

Product Reactivity/Detection: IL-1RI

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 34 citations in various scientific publications

Available Conjugates/Forms for IL-1RI Antibody (H-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin