Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
IL-1ra CRISPR/Cas9 KO Plasmid (h): sc-401313
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IL-1ra CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the IL-1ra genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IL-1ra Antibody (A-4): sc-374084
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IL-1ra CRISPR/Cas9 KO Plasmid (h) | sc-401313 | 20 µg | $397.00 |
Overview
Human IL1RN encodes interleukin-1 receptor antagonist (IL-1ra), a secreted regulator that competitively inhibits IL-1α and IL-1β binding to IL-1 receptor type I, thereby attenuating downstream MyD88-dependent signaling. By restraining IL-1–driven activation of NF-κB and MAPK pathways, IL-1ra modulates transcriptional programs controlling cytokine production, leukocyte recruitment, and acute-phase inflammatory responses. Dysregulated IL1RN activity is linked to aberrant innate immune signaling and altered inflammatory set points, making it relevant to studies of autoinflammation, arthritis-associated pathways, and broader cytokine network regulation. IL1RN is also used as a marker node for interrogating inflammasome-IL-1 axis dynamics and feedback control in macrophages, epithelial cells, and stromal compartments.
IL-1ra CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the IL1RN gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the IL1RN together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the IL1RN open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish IL-1ra protein expression.
This CRISPR knockout system enables efficient generation of IL1RN-deficient cell models for investigation of IL-1ra signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting IL1RN exon(s) critical for IL-1ra function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple IL1RN genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by IL-1ra CRISPR/Cas9 KO Plasmid (h) and IL-1ra CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the IL1RN locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by IL-1ra HDR Plasmid (h) and IL-1ra HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by IL1RN homology arms to support homology-directed repair at defined IL1RN target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.