IL-10Rα Antibody (E-6): sc-28371

IL-10Rα Antibody (E-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects IL-10Rα protein of mouse origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-IL-10Rα antibody (E-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. IL-10R is a crucial component of the immune system, belonging to the class II subgroup of the cytokine receptor family, and shares structural similarities with the interferon receptor. IL-10R is predominantly expressed in B cells and T helper cells, as well as in lipopolysaccharide-induced mouse fibroblasts, highlighting a role in immune response regulation. IL-10Rα is essential for mediating anti-inflammatory effects of interleukin-10 (IL-10), a cytokine that plays a significant role in limiting immune responses and preventing excessive inflammation. Upon IL-10 binding, IL-10R undergoes a conformational change that activates associated Janus kinases, JAK1 and Tyk2, leading to phosphorylation of specific tyrosine residues in the cytoplasmic domain of IL-10Rα. This post-translational modification is critical for IL-10R functionality and subsequent activation of IL-10 signaling pathways, which are vital for maintaining immune homeostasis. IL-10Rβ, which is ubiquitously expressed, forms heterodimeric receptors with both IL-22R and IL-28R subunits, expanding the functional repertoire of IL-10 signaling. IL-10R is also constitutively expressed on human natural killer (NK) cells, where direct binding of IL-10 enhances cytokine production, underscoring IL-10R importance in modulating immune responses.

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IL-10Rα Antibody (E-6) References:

1. Regulatory activity of autocrine IL-10 on dendritic cell functions.  |  Corinti, S., et al. 2001. J Immunol. 166: 4312-8. PMID: 11254683
2. Novel interferons.  |  Vilcek, J. 2003. Nat Immunol. 4: 8-9. PMID: 12496969
3. IL-10 signaling via IL-10E1 is dependent on tyrosine phosphorylation in the IL-10R alpha chain in human primary prostate cancer cell lines.  |  Stearns, ME., et al. 2003. Oncogene. 22: 3781-91. PMID: 12802285
4. Cutting edge: IL-26 signals through a novel receptor complex composed of IL-20 receptor 1 and IL-10 receptor 2.  |  Sheikh, F., et al. 2004. J Immunol. 172: 2006-10. PMID: 14764663
5. IL-10Rα expression is post-transcriptionally regulated by miR-15a, miR-185, and miR-211 in melanoma.  |  Venza, I., et al. 2015. BMC Med Genomics. 8: 81. PMID: 26631117
6. Exclusive dependence of IL-10Rα signalling on intestinal microbiota homeostasis and control of whipworm infection.  |  Duque-Correa, MA., et al. 2019. PLoS Pathog. 15: e1007265. PMID: 30640950
7. IL-23-producing IL-10Rα-deficient gut macrophages elicit an IL-22-driven proinflammatory epithelial cell response.  |  Bernshtein, B., et al. 2019. Sci Immunol. 4: PMID: 31201258
8. The functional characterization of interleukin-10 receptor expression on human natural killer cells.  |  Carson, WE., et al. 1995. Blood. 85: 3577-85. PMID: 7540068
9. Functional regions of the mouse interleukin-10 receptor cytoplasmic domain.  |  Ho, AS., et al. 1995. Mol Cell Biol. 15: 5043-53. PMID: 7544437
10. Characterization of recombinant extracellular domain of human interleukin-10 receptor.  |  Tan, JC., et al. 1995. J Biol Chem. 270: 12906-11. PMID: 7759550
11. Lipopolysaccharide-dependent induction of IL-10 receptor expression on murine fibroblasts.  |  Weber-Nordt, RM., et al. 1994. J Immunol. 153: 3734-44. PMID: 7930590
12. A receptor for interleukin 10 is related to interferon receptors.  |  Ho, AS., et al. 1993. Proc Natl Acad Sci U S A. 90: 11267-71. PMID: 8248239

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-IL-10Rα Antibody (E-6) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-IL-10Rα Antibody (E-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

IL-10Rα Antibody (E-6) Specifications

Alternate Product Name: IL-10Rα

Product Clone Name: E-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 276-575

Epitope Target: IL-10Rα

Epitope Species: mouse

Product Reactivity/Detection: IL-10Rα

Species Reactivity/Detection: mouse

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for IL-10Rα Antibody (E-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin