IKKγ Antibody (F-12): sc-166397

IKKγ Antibody (F-12) is a mouse monoclonal IgG1 antibody designed to detect full-length IKKγ protein, also known as NEMO (NF-κB Essential Modulator), across mouse, rat, and human species using western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF) techniques. IKKγ is a critical regulatory subunit of the IKK complex, which plays an essential role in the activation of the NF-κB signaling pathway, a key mediator of immune responses, inflammation, and cell survival. The structure of IKKγ includes multiple zinc finger motifs and a ubiquitin-binding domain, enabling interactions with other proteins such as IKKα, IKKβ, and the NF-κB inducing kinase (NIK). These interactions are vital for the phosphorylation and subsequent degradation of IκB inhibitors, allowing NF-κB to translocate to the nucleus and activate target genes. Post-translational modifications, particularly ubiquitination, regulate IKKγ′s activity and interactions with kinases and ubiquitin ligases, thereby modulating the intensity and duration of the NF-κB response. By facilitating these protein-protein interactions, IKKγ ensures precise control over cellular responses to proinflammatory cytokines and stress signals. Anti-IKKγ antibody (1.T.26) enables researchers to study these dynamic interactions and modifications, providing insights into the molecular mechanisms underlying diseases such as cancer, autoimmune disorders, and chronic inflammation. With high specificity and robust performance, IKKγ monoclonal antibody (1.T.26) is an indispensable tool for advancing research in molecular biology and immunology, supporting the elucidation of complex regulatory networks that govern cellular responses.

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IKKγ Antibody (F-12) References:

1. IKK regulation and human genetics.  |  Courtois, G. and Israël, A. 2011. Curr Top Microbiol Immunol. 349: 73-95. PMID: 20845108
2. Novel Phosphorylations of IKKγ/NEMO.  |  Lee, SH., et al. 2012. mBio. 3: e00411-12. PMID: 23131831
3. GSK-3β controls NF-kappaB activity via IKKγ/NEMO.  |  Medunjanin, S., et al. 2016. Sci Rep. 6: 38553. PMID: 27929056
4. TBK1 and IKKε prevent TNF-induced cell death by RIPK1 phosphorylation.  |  Lafont, E., et al. 2018. Nat Cell Biol. 20: 1389-1399. PMID: 30420664
5. Annexin 1-nuclear factor-κB-microRNA-26a regulatory pathway in the metastasis of non-small cell lung cancer.  |  Guan, X., et al. 2019. Thorac Cancer. 10: 665-675. PMID: 30756482
6. Human Genetic Diseases Linked to the Absence of NEMO: An Obligatory Somatic Mosaic Disorder in Male.  |  Pescatore, A., et al. 2022. Int J Mol Sci. 23: PMID: 35163099
7. Uterus globulin associated protein 1 (UGRP1) binds podoplanin (PDPN) to promote a novel inflammation pathway during Streptococcus pneumoniae infection.  |  Han, L., et al. 2022. Clin Transl Med. 12: e850. PMID: 35652821
8. IKKγ/NEMO Localization into Multivesicular Bodies.  |  Wackernagel, LM., et al. 2022. Int J Mol Sci. 23: PMID: 35743220
9. NEMO- and RelA-dependent NF-κB signaling promotes small cell lung cancer.  |  Koerner, L., et al. 2023. Cell Death Differ. 30: 938-951. PMID: 36653597
10. NEMO-Binding Domain/IKKγ Inhibitory Peptide Alleviates Neuronal Pyroptosis in Spinal Cord Injury by Inhibiting ASMase-Induced Lysosome Membrane Permeabilization.  |  Geng, Y., et al. 2024. Adv Sci (Weinh). 11: e2405759. PMID: 39225315

The preferred IKKγ antibody is IKKγ Antibody (B-3): sc-8032.