IKKβ Antibody (H-4): sc-8014

IKKβ Antibody (H-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects IKK beta of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHCP), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). IKKβ Antibody (H-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. IKKβ plays a crucial role in the regulation of the NF-kB signaling pathway, which is vital for controlling immune responses, inflammation, and cell survival. Specifically, IKKβ is responsible for phosphorylating the inhibitory protein IkB, leading to its degradation and the subsequent release and activation of NF-kB. This process is essential for the transcription of genes involved in immune and inflammatory responses, making IKKβ a key player in various physiological and pathological conditions, including cancer and autoimmune diseases. The functional IKK complex, which includes IKKα, IKKβ, and IKKγ (NEMO), is critical for the phosphorylation of IkB, and understanding the dynamics of this complex can provide insights into therapeutic targets for diseases associated with dysregulated NF-kB signaling.

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IKKβ Antibody (H-4) References:

1. IκB kinase β (IKKβ): Structure, transduction mechanism, biological function, and discovery of its inhibitors.  |  Zhang, J., et al. 2023. Int J Biol Sci. 19: 4181-4203. PMID: 37705738
2. IKKβ stabilizes Mitofusin 2 and suppresses doxorubicin cardiomyopathy.  |  Guberman, M., et al. 2024. Cardiovasc Res. 120: 164-173. PMID: 38165268
3. IKKβ deletion from CNS macrophages increases neuronal excitability and accelerates the onset of EAE, while from peripheral macrophages reduces disease severity.  |  Avloniti, M., et al. 2024. J Neuroinflammation. 21: 34. PMID: 38279130
4. Rel/NF-kappa B/I kappa B family: intimate tales of association and dissociation.  |  Verma, IM., et al. 1995. Genes Dev. 9: 2723-35. PMID: 7590248
5. NF-kappa B: a lesson in family values.  |  Thanos, D. and Maniatis, T. 1995. Cell. 80: 529-32. PMID: 7867060
6. CHUK, a new member of the helix-loop-helix and leucine zipper families of interacting proteins, contains a serine-threonine kinase catalytic domain.  |  Connelly, MA. and Marcu, KB. 1995. Cell Mol Biol Res. 41: 537-49. PMID: 8777433
7. MAP3K-related kinase involved in NF-kappaB induction by TNF, CD95 and IL-1.  |  Malinin, NL., et al. 1997. Nature. 385: 540-4. PMID: 9020361
8. Identification and characterization of an IkappaB kinase.  |  Régnier, CH., et al. 1997. Cell. 90: 373-83. PMID: 9244310
9. A cytokine-responsive IkappaB kinase that activates the transcription factor NF-kappaB.  |  DiDonato, JA., et al. 1997. Nature. 388: 548-54. PMID: 9252186
10. Tumor necrosis factor (TNF)-mediated kinase cascades: bifurcation of nuclear factor-kappaB and c-jun N-terminal kinase (JNK/SAPK) pathways at TNF receptor-associated factor 2.  |  Song, HY., et al. 1997. Proc Natl Acad Sci U S A. 94: 9792-6. PMID: 9275204
11. The IkappaB kinase complex (IKK) contains two kinase subunits, IKKalpha and IKKbeta, necessary for IkappaB phosphorylation and NF-kappaB activation.  |  Zandi, E., et al. 1997. Cell. 91: 243-52. PMID: 9346241
12. Complementation cloning of NEMO, a component of the IkappaB kinase complex essential for NF-kappaB activation.  |  Yamaoka, S., et al. 1998. Cell. 93: 1231-40. PMID: 9657155

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-IKKβ Antibody (H-4) has 86 citations in a variety of scientific publications.

Primary antibodies like Anti-IKKβ Antibody (H-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

IKKβ Antibody (H-4) Specifications

Alternate Product Name: IKK beta

Product Clone Name: H-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 470-755

Epitope Target: IKKβ

Epitope Species: human

Product Reactivity/Detection: IKKβ

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 86 citations in various scientific publications

Available Conjugates/Forms for IKKβ Antibody (H-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin