IKK beta Antibody (H-4) recommended for detection of IKKβ of human origin by WB, IP, IF, IHC(P), FCM and ELISA

IKKβ Antibody (H-4): sc-8014

IKKβ Antibody (H-4) is rated 4.7 out of 5 by 15.
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Datasheets

    • Anti-IKKβ Antibody (H-4) is a mouse monoclonal IgG2a κ IKKβ antibody, cited in 70 publications, provided at 200 µg/ml
    • raised against amino acids 470-755 mapping at the C-terminus of IKKβ of human origin
    • IKK beta Antibody (H-4) is recommended for detection of IKKβ of human origin by WB, IP, IF, IHC(P), FCM and ELISA
    • Anti-IKK beta Antibody (H-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 405 for IF, IHC(P) and FCM
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IKKβ (H-4): sc-8014.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for IKKβ Antibody (H-4) for WB and IHC(P) applications. This reagent is now offered in a bundle with IKKβ Antibody (H-4) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

What is the recommended positive control for IKK beta Antibody (H-4): sc-8014?

Asked by: Trav11
Thank you for your question. For IKK beta Antibody (H-4): sc-8014, we have several recommended positive controls, which include: HeLa Whole Cell Lysate (sc-2200), Jurkat Whole Cell Lysate (sc-2204), HL-60 Whole Cell Lysate (sc-2209), as well as a few others. If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support 9
Date published: 2017-01-18
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Rated 1 out of 5 by from Non detected We could not detect any bands in WB (dilution 1:500).
Date published: 2018-03-07
Rated 5 out of 5 by from recommend this antibody I had detected this antbody,I suggest loading 20ug sample, The antibody diluted 1:1000 for WB analyisis for turbot intestine tissue .
Date published: 2017-07-18
Rated 5 out of 5 by from Good antibody. It works well in WB at dilution 1:500. I got clear band and I recommend this antibody.
Date published: 2017-07-13
Rated 5 out of 5 by from band is wonderful The working dilution is 1:200, we get positive results in WB,
Date published: 2017-05-13
Rated 5 out of 5 by from Strong band Excellent band observed in human cell lines in western blot
Date published: 2017-04-22
Rated 5 out of 5 by from Good antibody for western blot I used this antibody to do western blot in different human cell lines including 293T, DU145, LNCaP. It worked well in all of the cell lines.
Date published: 2017-04-05
Rated 5 out of 5 by from Great product Strong data for multiple applications; including WB,IF,FCM and IHC
Date published: 2017-01-31
Rated 5 out of 5 by from Works Great! This antibody works great in western blot analysis of IKKβ expression in IKKβ-transfected and control COS cells.
Date published: 2016-12-23
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