IgM Antibody (R1/69): sc-53347
- IgM Antibody (R1/69) is a mouse monoclonal IgG1 κ, cited in 3 publications, provided at 200 µg/ml
- raised against human myeloid cells
- IgM Antibody (R1/69) is recommended for detection of IgM of human origin by WB, IP, IF and IHC(P)
- Anti-IgM Antibody (R1/69) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for IgM Antibody (R1/69) for WB and IHC(P) applications. These reagents are now offered in bundles with IgM Antibody (R1/69) (see ordering information below).
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IgM Antibody (R1/69) is a mouse monoclonal IgG1 kappa light chain antibody that detects IgM of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). IgM (R1/69) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Immunoglobulin M (IgM) plays a crucial role in the immune response as the first antibody produced by B cells upon activation, serving as a key player in the early stages of infection. IgM′s unique pentameric structure, which consists of five monomeric units linked by disulfide bonds, allows for high avidity binding to antigens, making IgM particularly effective in activating the complement system. This feature is vital for the opsonization and clearance of pathogens, as well as for the formation of immune complexes. While IgM is predominantly found in serum, IgM′s presence in the interstitium is limited due to size, which restricts diffusion. Additionally, the J chain associated with IgM facilitates IgM′s role as a secretory immunoglobulin, highlighting IgM′s importance in mucosal immunity. IgM (R1/69) monoclonal antibody is an essential tool for researchers studying immune responses, particularly in the context of early infection and antibody development.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
IgM Antibody (R1/69) References:
- Immunoglobulin heavy chain constant regions regulate immunity and tolerance to idiotypes of antibody variable regions. | Reitan, SK. and Hannestad, K. 2002. Proc Natl Acad Sci U S A. 99: 7588-93. PMID: 12032327
- Monoclonal antibodies specific for murine IgM. II. Activation of B lymphocytes by monoclonal antibodies specific for the four constant domains of IgM. | Leptin, M. 1985. Eur J Immunol. 15: 131-7. PMID: 2578964
- Positive IgM in Congenital CMV Infection. | Bilavsky, E., et al. 2017. Clin Pediatr (Phila). 56: 371-375. PMID: 28006975
- Role of Natural IgM Autoantibodies (IgM-NAA) and IgM Anti-Leukocyte Antibodies (IgM-ALA) in Regulating Inflammation. | Lobo, PI. 2017. Curr Top Microbiol Immunol. 408: 89-117. PMID: 28698955
- Expression, distribution and specificity of Fc receptors for IgM on murine B cells. | Mathur, A., et al. 1988. J Immunol. 141: 1855-62. PMID: 2971716
- Post-rituximab immunoglobulin M (IgM) hypogammaglobulinemia. | Kridin, K. and Ahmed, AR. 2020. Autoimmun Rev. 19: 102466. PMID: 31917267
- CD5L is a canonical component of circulatory IgM. | Oskam, N., et al. 2023. Proc Natl Acad Sci U S A. 120: e2311265120. PMID: 38055740
- CD5L associates with IgM via the J chain. | Wang, Y., et al. 2024. Nat Commun. 15: 8397. PMID: 39333069
- A marmoset T-lymphocyte protein related to defined human serum immunoglobulin and fragments. | Marchalonis, JJ. and Wang, AC. 1981. J Immunogenet. 8: 165-75. PMID: 6167643
- Nucleotide sequence of the constant region of a chicken mu heavy chain immunoglobulin mRNA. | Dahan, A., et al. 1983. Nucleic Acids Res. 11: 5381-9. PMID: 6310496
- Unusual sequences in the murine immunoglobulin mu-delta heavy-chain region. | Richards, JE., et al. Nature. 306: 483-7. PMID: 6417547
- Mapping of heavy chain genes for mouse immunoglobulins M and D. | Liu, CP., et al. 1980. Science. 209: 1348-53. PMID: 6774414
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IgM Antibody (R1/69) | sc-53347 | 200 µg/ml | $316.00 | |||
IgM Antibody (R1/69): m-IgG Fc BP-HRP Bundle | sc-528565 | 200 µg Ab; 10 µg BP | $354.00 | |||
IgM Antibody (R1/69): m-IgGκ BP-HRP Bundle | sc-520971 | 200 µg Ab, 40 µg BP | $354.00 | |||
IgM Antibody (R1/69): m-IgG1 BP-HRP Bundle | sc-542999 | 200 µg Ab; 20 µg BP | $354.00 | |||
IgM Antibody (R1/69) AC | sc-53347 AC | 500 µg/ml, 25% agarose | $416.00 | |||
IgM Antibody (R1/69) HRP | sc-53347 HRP | 200 µg/ml | $316.00 | |||
IgM Antibody (R1/69) FITC | sc-53347 FITC | 200 µg/ml | $330.00 | |||
IgM Antibody (R1/69) PE | sc-53347 PE | 200 µg/ml | $343.00 | |||
IgM Antibody (R1/69) Alexa Fluor® 488 | sc-53347 AF488 | 200 µg/ml | $357.00 | |||
IgM Antibody (R1/69) Alexa Fluor® 546 | sc-53347 AF546 | 200 µg/ml | $357.00 | |||
IgM Antibody (R1/69) Alexa Fluor® 594 | sc-53347 AF594 | 200 µg/ml | $357.00 | |||
IgM Antibody (R1/69) Alexa Fluor® 647 | sc-53347 AF647 | 200 µg/ml | $357.00 | |||
IgM Antibody (R1/69) Alexa Fluor® 680 | sc-53347 AF680 | 200 µg/ml | $357.00 | |||
IgM Antibody (R1/69) Alexa Fluor® 790 | sc-53347 AF790 | 200 µg/ml | $357.00 |