Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
IGFBP1 CRISPR/Cas9 KO Plasmid (m): sc-421061
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IGFBP1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the IGFBP1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IGFBP1 Antibody (H-5): sc-55474
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IGFBP1 CRISPR/Cas9 KO Plasmid (m) | sc-421061 | 20 µg | $397.00 |
Overview
Igfbp1 encodes insulin-like growth factor binding protein 1 (IGFBP1), a secreted regulator that binds IGF-I and IGF-II to modulate their bioavailability, receptor engagement, and downstream signaling. By shaping IGF axis activity, IGFBP1 influences PI3K–AKT and MAPK pathway outputs that govern metabolic homeostasis, cell survival, and proliferation. In mouse physiology, hepatic Igfbp1 expression is strongly responsive to nutritional and hormonal cues, linking insulin signaling, gluconeogenic programs, and systemic growth factor dynamics. Dysregulated IGFBP1 levels have been associated with metabolic stress states and altered IGF signaling contexts relevant to liver function and inflammation-related phenotypes.
IGFBP1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Igfbp1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Igfbp1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Igfbp1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish IGFBP1 protein expression.
This CRISPR knockout system enables efficient generation of Igfbp1-deficient cell models for investigation of IGFBP1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Igfbp1 exon(s) critical for IGFBP1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Igfbp1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by IGFBP1 CRISPR/Cas9 KO Plasmid (m) and IGFBP1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Igfbp1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by IGFBP1 HDR Plasmid (m) and IGFBP1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Igfbp1 homology arms to support homology-directed repair at defined Igfbp1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.