IgA Antibody (B-12) recommended for detection of IgA1 and IgA2 of mouse, rat and human origin by WB, IP, IF and ELISA

IgA Antibody (B-12): sc-166334

IgA Antibody (B-12) is rated 5.0 out of 5 by 1.
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Datasheets

    • IgA Antibody (B-12) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-300 mapping at the N-terminus of IgA of human origin
    • IgA Antibody (B-12) is recommended for detection of IgA1 and IgA2 of mouse, rat and human origin by WB, IP, IF and ELISA
    • IgA Antibody (B-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IgA (B-12): sc-166334.
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What is the recommended starting dilution for Western blot for sc-166334: IgA (B-12) monoclonal antibody?

Asked by: Germaine
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from Great Western Blot data of IgA1 expression in non Great Western Blot data of IgA1 expression in non-transfected and human IgA1 transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-08-27
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