Ig lambda chain Antibody (Rs4) recommended for detection of λ light chains of Ig of human origin by WB, IP and IF

Ig λ chain Antibody (Rs4): sc-51638

Ig λ chain Antibody (Rs4) is rated 4.0 out of 5 by 2.
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5
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4
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Datasheets

    • Ig λ chain Antibody (Rs4) is a mouse monoclonal IgG1 provided at 100 µg/ml
    • raised against λ light chains of human IgG
    • recommended for detection of λ light chains of Ig of human origin by WB, IP and IF
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I am using Ig λ chain (Rs4): sc-51638 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Trav11
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Strong bulbous signal observed at the expected Strong bulbous signal observed at the expected molecular weight for Ig chain protein by WB using U266 whole cell lysate.
Date published: 2015-02-13
Rated 4 out of 5 by from Positive detection of the Ig chain protein Positive detection of the Ig chain protein by WB using human PBL whole cell lysate. -SCBT QC
Date published: 2013-02-01
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