IFN-γRα Antibody (2E2): sc-12753

IFN-γRα Antibody (2E2) is a hamster monoclonal IgG antibody that detects IFN-γRα, also called interferon gamma receptor 1 or IFNGR1, in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). IFN-γRα Antibody (2E2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The IFN-γ receptor, which includes IFN-γRα, plays a crucial role in mediating the effects of interferon-gamma (IFN-γ), a key cytokine involved in immune responses. Upon binding of IFN-γ, the receptor activates Janus kinases JAK1 and JAK2, leading to the phosphorylation of transcription factors Stat1α and Stat1β, which then translocate to the nucleus to initiate the transcription of genes involved in antiviral and immunomodulatory responses. Notably, while IFN-γRα is essential for ligand binding, IFN-γRα requires the presence of IFN-γRβ to fully activate the signaling cascade necessary for the expression of IFN-γ-inducible genes. The IFN-γRβ chain, also known as AF-1, is critical for signal transduction and varies in length between species, being 332 amino acids in mouse and 337 amino acids in human. This intricate interplay between the receptor components underscores the importance of both chains in orchestrating a robust immune response, making IFN-γRα a vital target for research in immunology and therapeutic interventions.

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IFN-γRα Antibody (2E2) References:

1. The interferon gamma receptor 1 (IFNGR1) -56C/T gene polymorphism is associated with increased risk of early gastric carcinoma.  |  Canedo, P., et al. 2008. Gut. 57: 1504-8. PMID: 18593809
2. Mice deficient in interferon-gamma or interferon-gamma receptor 1 have distinct inflammatory responses to acute viral encephalomyelitis.  |  Lee, EY., et al. 2013. PLoS One. 8: e76412. PMID: 24204622
3. Novel Mutation of Interferon-γ Receptor 1 Gene Presenting as Early Life Mycobacterial Bronchial Disease.  |  Gutierrez, MJ., et al. 2016. J Investig Med High Impact Case Rep. 4: 2324709616675463. PMID: 27868075
4. IFN-γR1 defects: Mutation update and description of the IFNGR1 variation database.  |  van de Vosse, E. and van Dissel, JT. 2017. Hum Mutat. 38: 1286-1296. PMID: 28744922
5. Interferon-γ Receptor 1 and GluR1 upregulated in motor neurons of symptomatic hSOD1G93A mice.  |  Sengupta, S., et al. 2019. Eur J Neurosci. 49: 62-78. PMID: 30457201
6. Loss of Optineurin Drives Cancer Immune Evasion via Palmitoylation-Dependent IFNGR1 Lysosomal Sorting and Degradation.  |  Du, W., et al. 2021. Cancer Discov. 11: 1826-1843. PMID: 33627378
7. Interferon gamma upregulates the cytokine receptors IFNGR1 and TNFRSF1A in HT-29-MTX E12 cells.  |  Johnson, B., et al. 2022. Cytokine. 156: 155892. PMID: 35653895
8. Tumor-intrinsic RGS1 potentiates checkpoint blockade response via ATF3-IFNGR1 axis.  |  Wang, B., et al. 2023. Oncoimmunology. 12: 2279800. PMID: 38264343
9. Deregulation of interferon-gamma receptor 1 expression and its implications for lung adenocarcinoma progression.  |  Tecalco-Cruz, AC., et al. 2024. World J Clin Oncol. 15: 195-207. PMID: 38455133
10. RNF149 Destabilizes IFNGR1 in Macrophages to Favor Postinfarction Cardiac Repair.  |  Huang, CK., et al. 2024. Circ Res. 135: 518-536. PMID: 38989590

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-IFN-γRα Antibody (2E2) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-IFN-γRα Antibody (2E2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

IFN-γRα Antibody (2E2) Specifications

Alternate Product Name: CD119

Product Clone Name: 2E2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG

Amino Acids:

Epitope Target: IFN-γRα

Epitope Species: mouse

Product Reactivity/Detection: IFN-γRα

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, FCM

Vialing Information: Each vial contains 200 µg IgG in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for IFN-γRα Antibody (2E2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred IFN-γRα antibody is IFN-γRα Antibody (GIR-94): sc-12755.