IFI-35 Antibody (E-7): sc-514213

IFI-35 Antibody (E-7) is a mouse monoclonal IgG1 antibody that detects IFI-35 (Interferon-induced 35 kDa protein) in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). IFI-35 is a 286 amino acid protein that plays a crucial role in the cellular response to interferons, particularly IFN-α, by modulating the expression of various target genes. IFI-35 is primarily localized in the nucleus and is expressed in key cell types, including macrophages, fibroblasts, and epithelial cells. Notably, IFI-35 is a leucine zipper protein that can form homodimers; however, IFI-35 is unique among its family as it does not bind DNA. Upon stimulation by IFN-α, IFI-35 interacts with N-Myc-interacting protein (Nmi), leading to the formation of a high molecular weight complex that is believed to be integral to IFN-α signaling pathways and subsequent cellular responses. This interaction also protects IFI-35 from proteasomal degradation, ensuring its availability when needed during the immune response. Additionally, two isoforms of IFI-35 arise from alternative splicing, which may contribute to its functional diversity and regulatory mechanisms within the cell. Anti-IFI-35 antibody (E-7) is an essential tool for researchers studying the intricate roles of interferon signaling and its implications in various biological processes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

IFI-35 Antibody (E-7) References:

1. Interferon-alpha induces nmi-IFP35 heterodimeric complex formation that is affected by the phosphorylation of IFP35.  |  Zhou, X., et al. 2000. J Biol Chem. 275: 21364-71. PMID: 10779520
2. Complexity of inflammatory responses in endothelial cells and vascular smooth muscle cells determined by microarray analysis.  |  Bandman, O., et al. 2002. Ann N Y Acad Sci. 975: 77-90. PMID: 12538156
3. The PH domain containing protein CKIP-1 binds to IFP35 and Nmi and is involved in cytokine signaling.  |  Zhang, L., et al. 2007. Cell Signal. 19: 932-44. PMID: 17197158
4. CKIP-1: a scaffold protein and potential therapeutic target integrating multiple signaling pathways and physiological functions.  |  Nie, J., et al. 2013. Ageing Res Rev. 12: 276-81. PMID: 22878216
5. Interferon-γ upregulates expression of IFP35 gene in HeLa cells via interferon regulatory factor-1.  |  Yang, W., et al. 2012. PLoS One. 7: e50932. PMID: 23226549
6. Interferon-inducible protein IFI35 negatively regulates RIG-I antiviral signaling and supports vesicular stomatitis virus replication.  |  Das, A., et al. 2014. J Virol. 88: 3103-13. PMID: 24371060
7. A critical role of interferon-induced protein IFP35 in the type I interferon response in cells induced by foot-and-mouth disease virus (FMDV) protein 2C.  |  Zheng, W., et al. 2014. Arch Virol. 159: 2925-35. PMID: 25085622
8. Fish TRIM32 functions as a critical antiviral molecule against iridovirus and nodavirus.  |  Yu, Y., et al. 2017. Fish Shellfish Immunol. 60: 33-43. PMID: 27847343
9. NMI and IFP35 serve as proinflammatory DAMPs during cellular infection and injury.  |  Xiahou, Z., et al. 2017. Nat Commun. 8: 950. PMID: 29038465
10. Physical mapping, cloning, and identification of genes within a 500-kb region containing BRCA1.  |  Brown, MA., et al. 1995. Proc Natl Acad Sci U S A. 92: 4362-6. PMID: 7753812

The preferred IFI-35 antibody is IFI-35 Antibody (B-1): sc-393513.