Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
IFI-202 CRISPR/Cas9 KO Plasmid (m): sc-424028
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- IFI-202 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the IFI-202 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: IFI-202 Antibody (F-7): sc-166253
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IFI-202 CRISPR/Cas9 KO Plasmid (m) | sc-424028 | 20 µg | $397.00 |
Overview
Mouse Ifi202b encodes IFI-202, an interferon-inducible member of the p200 (PYHIN) family implicated in innate immune regulation and control of cell fate decisions. IFI-202 has been linked to modulation of DNA damage responses, cell-cycle progression, and apoptotic signaling, with functional connections to interferon/ISG networks that shape inflammatory gene expression. In immune cells and interferon-stimulated contexts, altered Ifi202b activity is associated with dysregulated immune homeostasis and has been studied in relation to autoimmune-like phenotypes and inflammatory disease mechanisms in mouse models.
IFI-202 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ifi202b gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ifi202b together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ifi202b open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish IFI-202 protein expression.
This CRISPR knockout system enables efficient generation of Ifi202b-deficient cell models for investigation of IFI-202 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ifi202b exon(s) critical for IFI-202 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ifi202b genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by IFI-202 CRISPR/Cas9 KO Plasmid (m) and IFI-202 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ifi202b locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by IFI-202 HDR Plasmid (m) and IFI-202 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ifi202b homology arms to support homology-directed repair at defined Ifi202b target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.