IEX-1 Antibody (E-2) recommended for detection of IEX-1 of mouse, rat origin by WB, IP, IF and ELISA

IEX-1 Antibody (E-2): sc-515605

IEX-1 Antibody (E-2) is rated 4.0 out of 5 by 1.
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Datasheets

    • Anti-IEX-1 Antibody (E-2) is a mouse monoclonal IgG1 (kappa light chain) IEX-1 antibody provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 3-25 at the N-terminus of IEX-1 of mouse origin
    • Anti-IEX-1 Antibody (E-2) is recommended for detection of IEX-1 of mouse and rat origin by WB, IP, IF and ELISA
    • Anti-IEX-1 Antibody (E-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IEX-1 (E-2): sc-515605.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for IEX-1 Antibody (E-2) for WB applications. This reagent is now offered in a bundle with IEX-1 Antibody (E-2) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

Do you know if the anti-IEX1 antibody (sc-515605) works to detect human IEX-1 in WB application?

Asked by: Anonymous
Thank you for your question. IEX-1 Antibody (E-2): sc-515605 is recommended for the detection of IEX-1 of mouse and rat origin by WB, IP, IF and ELISA. We do not recommend this antibody for detection of IEX-1 in human samples.
Answered by: Tech Support Europe
Date published: 2019-07-02

What application is the blocking peptide sc-515605 P appropriate for?

Asked by: Professor Griffin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-28
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Rated 4 out of 5 by from Works well for WB but not for IF or IHC Used at a dilution of 1:500 for 30ug protein on mouse neonatal lungs, with overnight incubation at 4oC
Date published: 2019-04-23
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