Anti-IEX-1 Antibody (E-2) is a mouse monoclonal IgG1 (kappa light chain) IEX-1 antibody provided at 200 µg/ml
specific for an epitope mapping between amino acids 3-25 at the N-terminus of IEX-1 of mouse origin
Anti-IEX-1 Antibody (E-2) is recommended for detection of IEX-1 of mouse and rat origin by WB, IP, IF and ELISA
Anti-IEX-1 Antibody (E-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of IEX-1 (E-2): sc-515605.
m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for IEX-1 Antibody (E-2) for WB applications. This reagent is now offered in a bundle with IEX-1 Antibody (E-2) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
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SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
IEX-1 Antibody (E-2) is a high quality monoclonal IEX-1 antibody (also designated IEX-1 antibody) suitable for the detection of the IEX-1 protein of mouse and rat origin. IEX-1 Antibody (E-2) is available as both the non-conjugated anti-IEX-1 antibody form, as well as multiple conjugated forms of anti-IEX-1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Tumors are frequently observed as resistant to apoptotic induction by FAS, tumor necrosis factor α (TNF-α) or irradiation. This anti-death activity may be attributed to immediate early-response genes that are regulated at the transcriptional level, including the protein IEX-1. IEX-1 (immediately early gene X-1), also known as IER3 (immediate early response 3), DIF-2 (differentiation-dependent gene 2 protein), immediate early protein GLY96 or PRG1 (PACAP-responsive gene 1 protein), is a 156 amino acid single-pass type II membrane protein that belongs to the IER3 family. IEX-1 was originally characterized as a gene induced by ultraviolet radiation and TNF-α, which protected human squamous carcinoma cells from apoptosis. Subsequent transfection studies have also shown that expression of IEX-1 in human keratinocytes and mouse fibroblasts results in more rapid proliferation of the cells as compared with controls. The promoter region of IEX-1 contains binding motifs for both NF-kB and p53, suggesting that these proteins may regulate its expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Do you know if the anti-IEX1 antibody (sc-515605) works to detect human IEX-1 in WB application?
Asked by: Anonymous
Thank you for your question. IEX-1 Antibody (E-2): sc-515605 is recommended for the detection of IEX-1 of mouse and rat origin by WB, IP, IF and ELISA. We do not recommend this antibody for detection of IEX-1 in human samples.
Answered by: Tech Support Europe
Date published: 2019-07-02
What application is the blocking peptide sc-515605 P appropriate for?
Asked by: Professor Griffin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here:
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn