IDS Antibody (B-5): sc-365047

IDS Antibody (B-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects IDS protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). IDS (B-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. IDS protein, also known as Iduronate 2-sulfatase or SIDS, is a 550 amino acid enzyme localized primarily in the lysosome, where IDS plays a crucial role in the degradation of glycosaminoglycans such as dermatan sulfate and heparan sulfate. This enzymatic function is vital for maintaining cellular homeostasis and preventing the accumulation of these complex carbohydrates, which can lead to cellular dysfunction. Mutations in IDS gene result in mucopolysaccharidosis type 2 (MPS2), commonly referred to as Hunter syndrome, a serious condition characterized by skeletal deformities, organ enlargement, progressive cardiopulmonary issues, and neurological impairment. Notably, IDS exists in two alternatively spliced isoforms, which may have implications for functional diversity and regulation within different tissues.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

IDS Antibody (B-5) References:

1. The 2.1-, 5.4- and 5.7-kb transcripts of the IDS gene are generated by different polyadenylation signals.  |  Cudry, S., et al. 1999. Biochim Biophys Acta. 1447: 35-42. PMID: 10500241
2. The effect of four mutations on the expression of iduronate-2-sulfatase in mucopolysaccharidosis type II.  |  Bonuccelli, G., et al. 2001. Biochim Biophys Acta. 1537: 233-8. PMID: 11731225
3. Expression studies of two novel in CIS-mutations identified in an intermediate case of Hunter syndrome.  |  Ricci, V., et al. 2003. Am J Med Genet A. 120A: 84-7. PMID: 12794697
4. General implications for CpG hot spot mutations: methylation patterns of the human iduronate-2-sulfatase gene locus.  |  Tomatsu, S., et al. 2004. Hum Mutat. 23: 590-8. PMID: 15146464
5. Analysis of normal and mutant iduronate-2-sulphatase conformation.  |  Parkinson-Lawrence, E., et al. 2005. Biochem J. 386: 395-400. PMID: 15500445
6. Effect of Hunter disease (mucopolysaccharidosis type II) mutations on molecular phenotypes of iduronate-2-sulfatase: enzymatic activity, protein processing and structural analysis.  |  Sukegawa-Hayasaka, K., et al. 2006. J Inherit Metab Dis. 29: 755-61. PMID: 17091340
7. Enigmatic in vivo iduronate-2-sulfatase (IDS) mutant transcript correction to wild-type in Hunter syndrome.  |  Lualdi, S., et al. 2010. Hum Mutat. 31: E1261-85. PMID: 20104590
8. In vitro recapitulation of the site-specific editing (to wild-type) of mutant IDS mRNA transcripts, and the characterization of IDS protein translated from the edited mRNAs.  |  Lualdi, S., et al. 2017. Hum Mutat. 38: 849-862. PMID: 28477385
9. Tagged IDS causes efficient and engraftment-independent prevention of brain pathology during lentiviral gene therapy for Mucopolysaccharidosis type II.  |  Catalano, F., et al. 2023. Mol Ther Methods Clin Dev. 31: 101149. PMID: 38033460
10. Sequence of the human iduronate 2-sulfatase (IDS) gene.  |  Wilson, PJ., et al. 1993. Genomics. 17: 773-5. PMID: 8244397
11. Identification of an alternative transcript from the human iduronate-2-sulfatase (IDS) gene.  |  Malmgren, H., et al. 1995. Genomics. 29: 291-3. PMID: 8530090
12. Molecular basis of iduronate-2-sulphatase gene mutations in patients with mucopolysaccharidosis type II (Hunter syndrome).  |  Li, P., et al. 1999. J Med Genet. 36: 21-7. PMID: 9950361

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-IDS Antibody (B-5) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-IDS Antibody (B-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

IDS Antibody (B-5) Specifications

Alternate Product Name: IDS

Product Clone Name: B-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 92-334

Epitope Target: IDS

Epitope Species: human

Product Reactivity/Detection: IDS

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for IDS Antibody (B-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin