IDH1 Antibody (F-3): sc-515396

IDH1 (F-3) is a mouse monoclonal IgM kappa antibody (also designated IDH antibody, PICD antibody or Oxalosuccinate decarboxylase antibody) that detects IDH1 protein of mouse, rat and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF) and enzyme-linked immunosorbent assay (ELISA). IDH1 (F-3) antibody is available as the non-conjugated anti-IDH1 antibody. The Isocitrate dehydrogenase (IDHC or IDH) cytoplasmic enzyme is a homodimer of 416 residues that belongs to the isocitrate and isopropylmalate dehydrogenases family. IDHC catalyzes the third step of the citric acid cycle, which involves the oxidative decarboxylation of isocitrate, forming α-ketoglutarate and CO2 in a two step reaction. The first step involves the oxidation of isocitrate to the intermediate oxalosuccinate, while the second step involves the production of α-ketoglutarate. During this process, either NADH or NADPH is produced along with CO2. Ca2+ can bind to IDHC as a complex with isocitrate, acting as a competitive inhibitor of Mg2+. The IDHC enzyme is inactivated by phosphorylation at Ser 113 and contains a clasp-like domain wherein both polypeptide chains in the dimer interlock. IDHC is expressed in a wide range of species and also in organisms that lack a complete citric acid cycle.

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IDH1 Antibody (F-3) References:

1. The human PICD gene encodes a cytoplasmic and peroxisomal NADP(+)-dependent isocitrate dehydrogenase.  |  Geisbrecht, BV. and Gould, SJ. 1999. J Biol Chem. 274: 30527-33. PMID: 10521434
2. Structures of human cytosolic NADP-dependent isocitrate dehydrogenase reveal a novel self-regulatory mechanism of activity.  |  Xu, X., et al. 2004. J Biol Chem. 279: 33946-57. PMID: 15173171
3. Modulation of TCA cycle enzymes and electron transport chain systems in experimental lung cancer.  |  Senthilnathan, P., et al. 2006. Life Sci. 78: 1010-4. PMID: 16143346
4. Comparison of Mycobacterium tuberculosis isocitrate dehydrogenases (ICD-1 and ICD-2) reveals differences in coenzyme affinity, oligomeric state, pH tolerance and phylogenetic affiliation.  |  Banerjee, S., et al. 2005. BMC Biochem. 6: 20. PMID: 16194279
5. Oxalomalate, a competitive inhibitor of NADP+-dependent isocitrate dehydrogenase, regulates heat shock-induced apoptosis.  |  Kim, HJ. and Park, JW. 2005. Biochem Biophys Res Commun. 337: 685-91. PMID: 16202391
6. Calcium overload increases oxidative stress in old rat gastrocnemius muscle.  |  Capel, F., et al. 2005. J Physiol Pharmacol. 56: 369-80. PMID: 16204760
7. Structure of a bacterial enzyme regulated by phosphorylation, isocitrate dehydrogenase.  |  Hurley, JH., et al. 1989. Proc Natl Acad Sci U S A. 86: 8635-9. PMID: 2682654
8. Inactivation of isocitrate dehydrogenase by phosphorylation is mediated by the negative charge of the phosphate.  |  Thorsness, PE. and Koshland, DE. 1987. J Biol Chem. 262: 10422-5. PMID: 3112144
9. Ivosidenib in Isocitrate Dehydrogenase 1-Mutated Advanced Glioma.  |  Mellinghoff, IK., et al. 2020. J Clin Oncol. 38: 3398-3406. PMID: 32530764
10. Ivosidenib and Azacitidine in IDH1-Mutated Acute Myeloid Leukemia.  |  Montesinos, P., et al. 2022. N Engl J Med. 386: 1519-1531. PMID: 35443108
11. Mutant IDH1 inhibition induces dsDNA sensing to activate tumor immunity.  |  Wu, MJ., et al. 2024. Science. 385: eadl6173. PMID: 38991060
12. Cytosolic isocitrate dehydrogenase in humans, mice, and voles and phylogenetic analysis of the enzyme family.  |  Nekrutenko, A., et al. 1998. Mol Biol Evol. 15: 1674-84. PMID: 9866202

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-IDH1 Antibody (F-3) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-IDH1 Antibody (F-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

IDH1 Antibody (F-3) Specifications

Alternate Product Name: IDH1

Product Clone Name: F-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 33-58

Epitope Target: IDH1

Epitope Species: human

Product Reactivity/Detection: IDH1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for IDH1 Antibody (F-3): P: Blocking Peptide

The preferred IDH1/2 antibody is IDH1/2 Antibody (G-11): sc-373816.