Id3 Antibody (4i234): sc-71311

Id3 Antibody (4i234) is a mouse monoclonal IgG1 antibody that detects Id3 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Id3 is a member of the Id family of basic helix-loop-helix (bHLH) proteins, which play a crucial role in regulating cell differentiation and proliferation. Specifically, Id3 functions as a negative regulator of DNA binding for bHLH proteins, inhibiting the activity of key transcription factors involved in muscle-specific gene expression, such as E12 and Myo D. This inhibition is particularly important during muscle cell differentiation; as Id3 levels decrease, E12 and/or E47 can form heterodimers with Myo D and myogenin, thereby promoting myogenic differentiation. Id3 expression is tightly regulated by growth factors, and studies have shown that reducing Id3 mRNA levels can delay the reentry of quiescent cells into the cell cycle following growth factor stimulation. This highlights Id3′s significance in cellular responses to external signals and potential implications in muscle development and regeneration. Anti-Id3 antibody (4i234) is an essential tool for researchers studying the intricate mechanisms of muscle differentiation and the broader roles of bHLH proteins in various biological processes.

The preferred Id1 antibody is Id1 Antibody (B-8): sc-133104.