Id2 Antibody (E-7): sc-398104

Id2 Antibody (E-7) is a mouse monoclonal IgG2a antibody that detects Id2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Id2 (E-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Id2 protein, a member of the basic helix-loop-helix (bHLH) family, plays a crucial role in regulating cell differentiation and proliferation by inhibiting DNA binding activity of other bHLH proteins. Such inhibition is particularly important in muscle development, as Id2 prevents binding of myogenic factors such as E12 and Myo D, thereby suppressing muscle-specific gene expression. As muscle cells differentiate, Id protein levels decrease, allowing E12 and/or E47 to form heterodimers with Myo D and myogenin, which are essential for activating myogenic differentiation. Expression of Id proteins, including Id2, is tightly regulated by growth factors, and studies have shown that reducing Id mRNA levels can delay reentry of quiescent cells into cell cycle following growth factor stimulation. This highlights Id2′s significance in cellular processes and potential as a target for research in developmental biology and cancer.

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Id2 Antibody (E-7) References:

1. Id proteins Id1 and Id2 selectively inhibit DNA binding by one class of helix-loop-helix proteins.  |  Sun, XH., et al. 1991. Mol Cell Biol. 11: 5603-11. PMID: 1922066
2. An Id-related helix-loop-helix protein encoded by a growth factor-inducible gene.  |  Christy, BA., et al. 1991. Proc Natl Acad Sci U S A. 88: 1815-9. PMID: 2000388
3. The protein Id: a negative regulator of helix-loop-helix DNA binding proteins.  |  Benezra, R., et al. 1990. Cell. 61: 49-59. PMID: 2156629
4. Id2 deletion attenuates Apc-deficient ileal tumor formation.  |  Biyajima, K., et al. 2015. Biol Open. 4: 993-1001. PMID: 26163528
5. Id2 Mediates Differentiation of Labyrinthine Placental Progenitor Cell Line, SM10.  |  Selesniemi, K., et al. 2016. Stem Cells Dev. 25: 959-74. PMID: 27168216
6. Id2 Determines Intestinal Identity through Repression of the Foregut Transcription Factor Irx5.  |  Mori, K., et al. 2018. Mol Cell Biol. 38: PMID: 29463648
7. An Id2RFP-Reporter Mouse Redefines Innate Lymphoid Cell Precursor Potentials.  |  Xu, W., et al. 2019. Immunity. 50: 1054-1068.e3. PMID: 30926235
8. Quercetin prevents the ferroptosis of OPCs by inhibiting the Id2/transferrin pathway.  |  Wang, Y., et al. 2023. Chem Biol Interact. 381: 110556. PMID: 37230155
9. HLH forced dimers: tethering MyoD to E47 generates a dominant positive myogenic factor insulated from negative regulation by Id.  |  Neuhold, LA. and Wold, B. 1993. Cell. 74: 1033-42. PMID: 7691411
10. The expression pattern of Id4, a novel dominant negative helix-loop-helix protein, is distinct from Id1, Id2 and Id3.  |  Riechmann, V., et al. 1994. Nucleic Acids Res. 22: 749-55. PMID: 8139914
11. Id proteins control growth induction in mammalian cells.  |  Barone, MV., et al. 1994. Proc Natl Acad Sci U S A. 91: 4985-8. PMID: 8197168
12. Id-related genes encoding helix-loop-helix proteins are required for G1 progression and are repressed in senescent human fibroblasts.  |  Hara, E., et al. 1994. J Biol Chem. 269: 2139-45. PMID: 8294468

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Id2 Antibody (E-7) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-Id2 Antibody (E-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Id2 Antibody (E-7) Specifications

Alternate Product Name: Id2

Product Clone Name: E-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a

Amino Acids: 105-134

Epitope Target: Id2

Epitope Species: mouse

Product Reactivity/Detection: Id2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for Id2 Antibody (E-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent

The preferred Id1 antibody is Id1 Antibody (B-8): sc-133104.