Anti-Id2 Antibody (E-7) recommended for detection of Id2 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-Id2 Antibody (E-7): sc-398104

Anti-Id2 Antibody (E-7) is rated 4.7 out of 5 by 7.
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Datasheets

    • Anti-Id2 Antibody (E-7) is a mouse monoclonal IgG2a Id2 antibody, cited in 3 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 105-134 at the C-terminus of Id2 of mouse origin
    • Anti-Id2 Antibody (E-7) is recommended for detection of Id2 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Anti-Id2 Antibody (E-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-398104 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Id2 (E-7): sc-398104.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for Id2 Antibody (E-7) for WB applications. These reagents are now offered in bundles with Id2 Antibody (E-7) (see ordering information below).
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can it used for pig?

Asked by: huarenwu
Thank you for your question. We have not yet tested this antibody, Id2 (E-7): sc-398104, for use in pig samples so we do not recommend it for use with this species at this time. The antibody is raised against amino acids 105-134 at the C-terminus of Id2 of mouse origin. Based on sequence analysis, this sequence shares 90% sequence identity with Id2 of pig origin (protein accession number Q2VIU1). This is a high degree of identity, so there is a good chance that the antibody may also cross-react with pig.
Answered by: Technical Support
Date published: 2017-09-13

What application is the blocking peptide sc-398104 P appropriate for?

Asked by: Cweed
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Good antibody for Id2 detection by Western blot We tested this antibody on differentiating N1E-115 mouse cell line. It gives a clear signal at about 20 kDa, in line with the expected molecular weight of the protein (17,5 kDa). Background is very minimal. Antibody dilution: 1:250 in PBST with 5% nonfat dry milk.
Date published: 2021-02-17
Rated 4 out of 5 by from some background, but strong signal in WB diluted 1:100 in TBST, 5% BSA the AB detects endogenous and recombinant fusion protein in WB in NSCLC cell lines with some, yet weaker background
Date published: 2017-11-18
Rated 5 out of 5 by from Antibody effect is very good we are using renal tubular epithelial cells, with IF detection of Id2 expression, dilution ratio is 1: 80, the results are very good,Fluorescence is shown in the nucleus,antibody effect is very good.
Date published: 2017-10-12
Rated 5 out of 5 by from Great features Antibody effect is very good, we are using HT22 cells, with western blot detection of Id2 expression, dilution ratio is 1: 500, the results are very good.
Date published: 2017-07-17
Rated 5 out of 5 by from For therapy The Id2 antibody we used was mouse monoclonal IgG2a provided at 200 μg / ml, as suggested by WB, IP, IF, and ELISA as shown in the figure, and we performed a perfect procedure for targeted therapy
Date published: 2017-02-09
Rated 5 out of 5 by from Versatile and Clean Antibody This antibody is well-cited for a variety of applications including WB, IP, IF, and even ELISA. Strong, clear signal with little to no background. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-01-27
Rated 5 out of 5 by from Produced positive Western blot data of Id2 Produced positive Western blot data of Id2 expression in non-transfected and mouse Id2 transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-09-07
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