ICAM-1/CD54 Antibody (P2A4): sc-18839

ICAM-1 Antibody (P2A4) is a mouse monoclonal IgG1 kappa light chain antibody raised against IL-1β activated human vascular endothelial cells expressing ICAM-1, demonstrating reactivity with human species. Anti-ICAM-1 antibody (P2A4) is suitable for applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). ICAM-1, also known as CD54 or intercellular adhesion molecule-1, is a type I transmembrane glycoprotein belonging to the immunoglobulin superfamily. ICAM-1 plays a crucial role in immune responses by mediating leukocyte adhesion and transmigration across the endothelium during inflammation. ICAM-1 undergoes post-translational modifications such as N-linked glycosylation, which are important for stability, cell surface expression, and interaction with integrins like LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) on leukocytes. These modifications can affect ICAM-1′s function in cell adhesion and signaling pathways. Altered expression or glycosylation patterns of ICAM-1 are associated with various pathological conditions, including autoimmune diseases, cardiovascular disorders, and cancer metastasis. Understanding ICAM-1′s role and post-translational modifications is essential for research into inflammatory processes and potential therapeutic interventions.

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Contains Mercury: All HRP conjugates (including bundle forms) contain thiomersal, a mercury compound. Do not dispose in trash. Dispose as hazardous chemical waste according to local, state and federal regulations.

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ICAM-1/CD54 Antibody (P2A4) References:

1. ICAM-1: targeted docking for exogenous as well as endogenous ligands.  |  Hopkins, AM., et al. 2004. Adv Drug Deliv Rev. 56: 763-78. PMID: 15063588
2. Leukocyte rolling and adhesion via ICAM-1 signals to endothelial permeability. Focus on 'Leukocyte rolling and adhesion both contribute to regulation of microvascular permeability to albumin via ligation of ICAM-1'.  |  Williams, MR. and Luscinskas, FW. 2011. Am J Physiol Cell Physiol. 301: C777-9. PMID: 21775704
3. ICAM-1 and nanomedicine: nature's doorway to the extravascular tissue realm.  |  Lanza, GM. 2012. Arterioscler Thromb Vasc Biol. 32: 1070-1. PMID: 22517363
4. ICAM-1-dependent and ICAM-1-independent neutrophil lung infiltration by porcine reproductive and respiratory syndrome virus infection.  |  Liu, J., et al. 2015. Am J Physiol Lung Cell Mol Physiol. 309: L226-36. PMID: 26001774
5. NSun2 Deficiency Protects Endothelium From Inflammation via mRNA Methylation of ICAM-1.  |  Luo, Y., et al. 2016. Circ Res. 118: 944-56. PMID: 26838785
6. ADAM10-Mediated Cleavage of ICAM-1 Is Involved in Neutrophil Transendothelial Migration.  |  Morsing, SKH., et al. 2021. Cells. 10: PMID: 33504031
7. ImmunoPET/NIRF/Cerenkov multimodality imaging of ICAM-1 in pancreatic ductal adenocarcinoma.  |  Li, M., et al. 2021. Eur J Nucl Med Mol Imaging. 48: 2737-2748. PMID: 33537836
8. ICAM-1 Activates Platelets and Promotes Endothelial Permeability through VE-Cadherin after Insufficient Radiofrequency Ablation.  |  Kong, J., et al. 2021. Adv Sci (Weinh). 8: 2002228. PMID: 33643788
9. ICAM-1 induced rearrangements of capsid and genome prime rhinovirus 14 for activation and uncoating.  |  Hrebík, D., et al. 2021. Proc Natl Acad Sci U S A. 118: PMID: 33947819
10. Oligodendrocytes regulate the adhesion molecule ICAM-1 in neuroinflammation.  |  González-Alvarado, MN., et al. 2022. Glia. 70: 522-535. PMID: 34787332

The preferred ICAM-1/CD54 antibody is ICAM-1/CD54 Antibody (G-5): sc-8439.