Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
I2PP2A CRISPR/Cas9 KO Plasmid (h): sc-400881
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- I2PP2A CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the I2PP2A genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: I2PP2A Antibody (F-9): sc-133138
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
I2PP2A CRISPR/Cas9 KO Plasmid (h) | sc-400881 | 20 µg | $397.00 |
Overview
SET (also known as I2PP2A) encodes a multifunctional nuclear and cytoplasmic protein that inhibits protein phosphatase 2A (PP2A), thereby influencing phosphorylation-dependent control of cell cycle progression, apoptosis, and transcriptional programs. I2PP2A participates in chromatin regulation through interactions with histones and chromatin-associated complexes, linking SET to DNA damage responses, replication stress, and epigenetic modulation of gene expression. By restraining PP2A activity, SET can impact key signaling axes such as MAPK/ERK and PI3K/AKT that depend on balanced phosphorylation turnover. Dysregulated SET expression or activity has been associated with oncogenic signaling states and neuroinflammatory or neurodegenerative processes, making it a useful target for mechanistic studies of phosphatase regulation and chromatin-associated pathways.
I2PP2A CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SET gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SET together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SET open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish I2PP2A protein expression.
This CRISPR knockout system enables efficient generation of SET-deficient cell models for investigation of I2PP2A signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SET exon(s) critical for I2PP2A function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SET genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by I2PP2A CRISPR/Cas9 KO Plasmid (h) and I2PP2A CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SET locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by I2PP2A HDR Plasmid (h) and I2PP2A HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SET homology arms to support homology-directed repair at defined SET target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.