I2PP2A Antibody (F-9): sc-133138

I2PP2A Antibody (F-9) is a mouse monoclonal IgG2a kappa light chain antibody that detects I2PP2A protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-I2PP2A antibody (F-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. I2PP2A protein, also known as inhibitor of protein phosphatase 2A, plays a crucial role in regulating cellular processes by selectively inhibiting the activity of the major serine/threonine phosphatase, protein phosphatase 2A (PP2A). This inhibition is significant because PP2A is involved in various signaling pathways that control cell growth, division, and apoptosis, making I2PP2A a key player in cellular regulation and a potential target for cancer therapies. Notably, I2PP2A is predominantly localized in the nucleus, where I2PP2A is highly expressed in Wilms′ tumor cells, suggesting involvement in tumorigenesis. Furthermore, transient expression of I2PP2A in HEK-293 cells has been shown to enhance DNA binding activity of proto-oncogene c-Jun, linking I2PP2A to oncogenic signaling pathways.

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I2PP2A Antibody (F-9) References:

1. Expression of I2PP2A, an inhibitor of protein phosphatase 2A, induces c-Jun and AP-1 activity.  |  Al-Murrani, SW., et al. 1999. Biochem J. 341 (Pt 2): 293-8. PMID: 10393085
2. Involvement of I2PP2A in the abnormal hyperphosphorylation of tau and its reversal by Memantine.  |  Chohan, MO., et al. 2006. FEBS Lett. 580: 3973-9. PMID: 16806196
3. Oncogenic Role of SET/I2PP2A for Gynecologic Cancers.  |  Jiang, SW., et al. 2017. Curr Drug Targets. 18: 1152-1157. PMID: 28356029
4. Distribution of SET/I2PP2A protein in gastrointestinal tissues.  |  Umata, K., et al. 2019. PLoS One. 14: e0222845. PMID: 31557212
5. Thirty years of SET/TAF1β/I2PP2A: from the identification of the biological functions to its implications in cancer and Alzheimer's disease.  |  Di Mambro, A. and Esposito, MT. 2022. Biosci Rep. 42: PMID: 36345878
6. Autophosphorylation-activated protein kinase inactivates the protein tyrosine phosphatase activity of protein phosphatase 2A.  |  Damuni, Z., et al. 1994. FEBS Lett. 352: 311-4. PMID: 7523189
7. Purification and characterization of two potent heat-stable protein inhibitors of protein phosphatase 2A from bovine kidney.  |  Li, M., et al. 1995. Biochemistry. 34: 1988-96. PMID: 7531497
8. Autophosphorylation-activated protein kinase phosphorylates and inactivates protein phosphatase 2A.  |  Guo, H. and Damuni, Z. 1993. Proc Natl Acad Sci U S A. 90: 2500-4. PMID: 7681598
9. The myeloid leukemia-associated protein SET is a potent inhibitor of protein phosphatase 2A.  |  Li, M., et al. 1996. J Biol Chem. 271: 11059-62. PMID: 8626647
10. Molecular identification of I1PP2A, a novel potent heat-stable inhibitor protein of protein phosphatase 2A.  |  Li, M., et al. 1996. Biochemistry. 35: 6998-7002. PMID: 8679524
11. I1PP2A and I2PP2A. Two potent protein phosphatase 2A-specific inhibitor proteins.  |  Li, M. and Damuni, Z. 1998. Methods Mol Biol. 93: 59-66. PMID: 9664527
12. Expression of SET, an inhibitor of protein phosphatase 2A, in renal development and Wilms' tumor.  |  Carlson, SG., et al. 1998. J Am Soc Nephrol. 9: 1873-80. PMID: 9773788

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-I2PP2A Antibody (F-9) has 19 citations in a variety of scientific publications.

Primary antibodies like Anti-I2PP2A Antibody (F-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

I2PP2A Antibody (F-9) Specifications

Alternate Product Name: SET nuclear proto-oncogene

Product Clone Name: F-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-120

Epitope Target: I2PP2A

Epitope Species: human

Product Reactivity/Detection: I2PP2A

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 19 citations in various scientific publications

Available Conjugates/Forms for I2PP2A Antibody (F-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin