HXK II Antibody (B-8): sc-374091

HXK II Antibody (B-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects HXK II protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-HXK II antibody (B-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HXK II plays a crucial role in glucose metabolism, catalyzing the conversion of glucose to glucose-6-phosphate, which is the first step in the glycolytic pathway. This enzymatic activity is vital for cellular energy production and metabolic regulation, particularly in tissues that rely heavily on glucose, such as adipose and skeletal muscle. HXK II is one of four hexokinase isoenzymes, each with distinct tissue distributions and regulatory mechanisms. While HXK I is expressed in brain, kidney, and heart tissues, HXK II is predominantly found in skeletal muscle and adipose tissue, where HXK II facilitates glucose uptake in response to insulin. The regulation of HXK II activity is essential for maintaining glucose homeostasis, and dysfunction is associated with metabolic disorders, including diabetes. Using HXK II monoclonal antibody (B-8), researchers can gain insights into the expression and regulation of this important enzyme in various physiological and pathological contexts.

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HXK II Antibody (B-8) References:

1. Human glucokinase gene: isolation, characterization, and identification of two missense mutations linked to early-onset non-insulin-dependent (type 2) diabetes mellitus.  |  Stoffel, M., et al. 1992. Proc Natl Acad Sci U S A. 89: 7698-702. PMID: 1502186
2. Glucose phosphorylation in tumor cells. Cloning, sequencing, and overexpression in active form of a full-length cDNA encoding a mitochondrial bindable form of hexokinase.  |  Arora, KK., et al. 1990. J Biol Chem. 265: 6481-8. PMID: 2318862
3. Hexokinase 2 as a novel selective metabolic target for rheumatoid arthritis.  |  Bustamante, MF., et al. 2018. Ann Rheum Dis. 77: 1636-1643. PMID: 30061164
4. Microbial regulation of hexokinase 2 links mitochondrial metabolism and cell death in colitis.  |  Hinrichsen, F., et al. 2021. Cell Metab. 33: 2355-2366.e8. PMID: 34847376
5. Upregulated hexokinase 2 expression induces the apoptosis of dopaminergic neurons by promoting lactate production in Parkinson's disease.  |  Li, J., et al. 2022. Neurobiol Dis. 163: 105605. PMID: 34973450
6. CRISPR/Cas9 Screens Reveal that Hexokinase 2 Enhances Cancer Stemness and Tumorigenicity by Activating the ACSL4-Fatty Acid β-Oxidation Pathway.  |  Li, H., et al. 2022. Adv Sci (Weinh). 9: e2105126. PMID: 35603967
7. The metabolic enzyme hexokinase 2 localizes to the nucleus in AML and normal haematopoietic stem and progenitor cells to maintain stemness.  |  Thomas, GE., et al. 2022. Nat Cell Biol. 24: 872-884. PMID: 35668135
8. A non-metabolic function of hexokinase 2 in small cell lung cancer: promotes cancer cell stemness by increasing USP11-mediated CD133 stability.  |  Wang, J., et al. 2022. Cancer Commun (Lond). 42: 1008-1027. PMID: 35975322
9. Research progress of hexokinase 2 in inflammatory-related diseases and its inhibitors.  |  Chen, J., et al. 2024. Eur J Med Chem. 264: 115986. PMID: 38011767
10. Multiple forms of hexokinase in the rat: tissue distribution, age dependency, and properties.  |  Katzen, HM. and Schimke, RT. 1965. Proc Natl Acad Sci U S A. 54: 1218-25. PMID: 5219826
11. Human hexokinase II: sequence and homology to other hexokinases.  |  Deeb, SS., et al. 1993. Biochem Biophys Res Commun. 197: 68-74. PMID: 8250948
12. Purification and characterization of the carboxyl-domain of human hexokinase type III expressed as fusion protein.  |  Palma, F., et al. 1996. Mol Cell Biochem. 155: 23-9. PMID: 8717435

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HXK II Antibody (B-8) has 42 citations in a variety of scientific publications.

Primary antibodies like Anti-HXK II Antibody (B-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HXK II Antibody (B-8) Specifications

Alternate Product Name: Hexokinase II

Product Clone Name: B-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 316-410

Epitope Target: HXK II

Epitope Species: human

Product Reactivity/Detection: HXK II

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 42 citations in various scientific publications

Available Conjugates/Forms for HXK II Antibody (B-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred HXK II antibody is HXK II Antibody (1A7): sc-130358.