HURP Antibody (E-7): sc-377004

HURP Antibody (E-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects HURP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). HURP (E-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HURP, also known as Hepatoma up-regulated protein or DLGAP5, is an 846 amino acid protein that plays a crucial role in cell cycle regulation, particularly during the G2/M phase. HURP localization to spindle poles in mitotic cells is vital for proper chromosome segregation, making HURP essential for maintaining genomic stability. HURP is expressed in various tissues, including the testis, colon, bone marrow, placenta, and fetal liver, and interacts with Cdc2 p34, which is important for mediating adherens junction assembly and differentiation in epithelial cells. HURP phosphorylation by ATM or ATR in response to DNA damage highlights HURP involvement in cellular stress responses, while HURP regulation through ubiquitin-induced proteasomal degradation ensures that HURP levels are tightly controlled. Two isoforms of HURP exist due to alternative splicing, which may contribute to HURP functional diversity in different cellular contexts.

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HURP Antibody (E-7) References:

1. Characterization of a novel human cell-cycle-regulated homologue of Drosophila dlg1.  |  Bassal, S., et al. 2001. Genomics. 77: 5-7. PMID: 11543626
2. Potential molecular marker for detecting transitional cell carcinoma.  |  Chiu, AW., et al. 2002. Urology. 60: 181-5. PMID: 12100959
3. Prognostic significance of hepatoma-up-regulated protein expression in patients with urinary bladder transitional cell carcinoma.  |  Huang, YL., et al. 2003. Anticancer Res. 23: 2729-33. PMID: 12894566
4. HURP is a Ran-importin beta-regulated protein that stabilizes kinetochore microtubules in the vicinity of chromosomes.  |  Silljé, HH., et al. 2006. Curr Biol. 16: 731-42. PMID: 16631580
5. HURP is part of a Ran-dependent complex involved in spindle formation.  |  Koffa, MD., et al. 2006. Curr Biol. 16: 743-54. PMID: 16631581
6. HURP controls spindle dynamics to promote proper interkinetochore tension and efficient kinetochore capture.  |  Wong, J. and Fang, G. 2006. J Cell Biol. 173: 879-91. PMID: 16769820
7. 'HURP on' we're off to the kinetochore!  |  Wilde, A. 2006. J Cell Biol. 173: 829-31. PMID: 16785318
8. HURP wraps microtubule ends with an additional tubulin sheet that has a novel conformation of tubulin.  |  Santarella, RA., et al. 2007. J Mol Biol. 365: 1587-95. PMID: 17118403
9. Gene expression analysis of hematopoietic progenitor cells identifies Dlg7 as a potential stem cell gene.  |  Gudmundsson, KO., et al. 2007. Stem Cells. 25: 1498-506. PMID: 17322106
10. Hurp deficiency in mice leads to female infertility caused by an implantation defect.  |  Tsai, CY., et al. 2008. J Biol Chem. 283: 26302-6. PMID: 18676373
11. HURP regulates chromosome congression by modulating kinesin Kif18A function.  |  Ye, F., et al. 2011. Curr Biol. 21: 1584-91. PMID: 21924616
12. Spindle-Length-Dependent HURP Localization Allows Centrosomes to Control Kinetochore-Fiber Plus-End Dynamics.  |  Dudka, D., et al. 2019. Curr Biol. 29: 3563-3578.e6. PMID: 31668617

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HURP Antibody (E-7) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-HURP Antibody (E-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HURP Antibody (E-7) Specifications

Alternate Product Name: HURP

Product Clone Name: E-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 417-451

Epitope Target: HURP

Epitope Species: human

Product Reactivity/Detection: HURP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for HURP Antibody (E-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin