HuR Antibody (G-5) recommended for detection of HuR, HuB, HuC and HuD of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

HuR Antibody (G-5): sc-374285

HuR Antibody (G-5) is rated 5.0 out of 5 by 2.
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Datasheets

    • HuR Antibody (G-5) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-280 of HuR of human origin
    • recommended for detection of HuR, HuB, HuC and HuD of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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I am using HuR (G-5): sc-374285 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: DefinitelyNotMatt
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Exceptional immunoperoxidase nuclear and Exceptional immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human rectum tissue. -SCBT QC
Date published: 2015-05-18
Rated 5 out of 5 by from Worked in WB in K Worked in WB in K-562 and HeLa whole cell lysates. -SCBT QC
Date published: 2015-02-13
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