Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HuC CRISPR/Cas9 KO Plasmid (h): sc-400172
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HuC CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HuC genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HuC Antibody (D-6): sc-515624
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HuC CRISPR/Cas9 KO Plasmid (h) | sc-400172 | 20 µg | $397.00 |
Overview
ELAVL3 (HuC) is a neuron-enriched RNA-binding protein of the Hu/ELAV family that recognizes AU-rich elements and regulates mRNA stability, splicing, localization, and translation in developing and mature neurons. By controlling post-transcriptional programs linked to neuronal differentiation, synaptic function, and neurite outgrowth, ELAVL3 helps shape activity-dependent gene expression and neuronal homeostasis. Altered regulation of ELAV-family RNA networks has been associated with neurodevelopmental and neurodegenerative processes, and ELAVL3 is frequently used as a marker and mechanistic entry point for studying neuronal identity. Mapping ELAVL3-dependent RNA regulons supports research into RNA metabolism pathways that influence excitability, synaptic plasticity, and stress responses in human neuronal models.
HuC CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ELAVL3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ELAVL3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ELAVL3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HuC protein expression.
This CRISPR knockout system enables efficient generation of ELAVL3-deficient cell models for investigation of HuC signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ELAVL3 exon(s) critical for HuC function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ELAVL3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HuC CRISPR/Cas9 KO Plasmid (h) and HuC CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ELAVL3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HuC HDR Plasmid (h) and HuC HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ELAVL3 homology arms to support homology-directed repair at defined ELAVL3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.