HtrA Antibody (B-8): sc-377050

HtrA Antibody (B-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects HtrA protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-HtrA antibody (B-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HtrA protein, a member of the serine protease family, plays a crucial role in degrading denatured proteins and regulating cell growth, making HtrA essential for maintaining cellular homeostasis. HtrA protein helps prevent misfolded protein accumulation during cellular stress, which can lead to cellular dysfunction. HtrA is secreted and expressed in various tissues, including osteoarthritic cartilage, and HtrA gene is highly conserved across mammalian species. Human HtrA2, also known as Omi, is a closely related protease that exhibits similar functions and participates in degrading aberrantly folded proteins. HtrA2 is expressed in the nucleus and exists in two polypeptide forms, including an alternatively spliced variant called D-Omi, which is predominantly found in kidney, colon, and thyroid. HtrA2′s unique structure, including a modified PDZ domain, allows specific partner interactions, although D-Omi does not bind to known partner Mxi2. HtrA and HtrA2′s ability to manage protein quality control under stress conditions underscores their significance in cellular health and disease.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

HtrA Antibody (B-8) References:

1. Expression, purification, and functional analysis of the human serine protease HtrA2.  |  Savopoulos, JW., et al. 2000. Protein Expr Purif. 19: 227-34. PMID: 10873535
2. Characterization of human HtrA2, a novel serine protease involved in the mammalian cellular stress response.  |  Gray, CW., et al. 2000. Eur J Biochem. 267: 5699-710. PMID: 10971580
3. Tissue-specific splicing of Omi stress-regulated endoprotease leads to an inactive protease with a modified PDZ motif.  |  Faccio, L., et al. 2000. Genomics. 68: 343-7. PMID: 10995577
4. The HtrA family of proteases: implications for protein composition and cell fate.  |  Clausen, T., et al. 2002. Mol Cell. 10: 443-55. PMID: 12408815
5. HtrA serine proteases as potential therapeutic targets in cancer.  |  Chien, J., et al. 2009. Curr Cancer Drug Targets. 9: 451-68. PMID: 19519315
6. Architecture and regulation of HtrA-family proteins involved in protein quality control and stress response.  |  Hansen, G. and Hilgenfeld, R. 2013. Cell Mol Life Sci. 70: 761-75. PMID: 22806565
7. Structural insights into the activation mechanisms of human HtrA serine proteases.  |  Zurawa-Janicka, D., et al. 2017. Arch Biochem Biophys. 621: 6-23. PMID: 28396256
8. HTRA family proteins in pregnancy outcome.  |  Fantone, S., et al. 2021. Tissue Cell. 72: 101549. PMID: 33915357
9. HtrA serine proteases in cancers: A target of interest for cancer therapy.  |  Wu, L., et al. 2021. Biomed Pharmacother. 139: 111603. PMID: 34243596
10. HtrA-Dependent E-Cadherin Shedding Impairs the Epithelial Barrier Function in Primary Gastric Epithelial Cells and Gastric Organoids.  |  Canadas-Ortega, M., et al. 2024. Int J Mol Sci. 25: PMID: 39000189
11. Primary structure of a putative serine protease specific for IGF-binding proteins.  |  Zumbrunn, J. and Trueb, B. 1996. FEBS Lett. 398: 187-92. PMID: 8977104
12. Human HtrA, an evolutionarily conserved serine protease identified as a differentially expressed gene product in osteoarthritic cartilage.  |  Hu, SI., et al. 1998. J Biol Chem. 273: 34406-12. PMID: 9852107

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HtrA Antibody (B-8) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-HtrA Antibody (B-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HtrA Antibody (B-8) Specifications

Alternate Product Name: HtrA

Product Clone Name: B-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 386-480

Epitope Target: HtrA

Epitope Species: human

Product Reactivity/Detection: HtrA

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for HtrA Antibody (B-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin