Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HspBP1 CRISPR/Cas9 KO Plasmid (h): sc-406487
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HspBP1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HspBP1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HspBP1 Antibody (F-11): sc-390467
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HspBP1 CRISPR/Cas9 KO Plasmid (h) | sc-406487 | 20 µg | $397.00 |
Overview
HSPBP1 encodes HspBP1, a nucleotide exchange factor and co-chaperone that regulates the HSP70/HSC70 chaperone cycle by modulating ATP/ADP exchange and client release. Through its interactions with molecular chaperones, HspBP1 contributes to proteostasis, stress response signaling, and the quality control of misfolded or damaged proteins that may otherwise accumulate and perturb cellular homeostasis. Dysregulation of chaperone networks, including HSP70 cofactor balance, is frequently linked to altered protein turnover and stress tolerance in contexts such as neurodegeneration and cancer biology, making HSPBP1 a useful node for mechanistic studies. In human cells, perturbing HspBP1 can help define how chaperone-assisted folding intersects with protein degradation pathways and stress-adaptive remodeling.
HspBP1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the HSPBP1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the HSPBP1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the HSPBP1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HspBP1 protein expression.
This CRISPR knockout system enables efficient generation of HSPBP1-deficient cell models for investigation of HspBP1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting HSPBP1 exon(s) critical for HspBP1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple HSPBP1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HspBP1 CRISPR/Cas9 KO Plasmid (h) and HspBP1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the HSPBP1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HspBP1 HDR Plasmid (h) and HspBP1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by HSPBP1 homology arms to support homology-directed repair at defined HSPBP1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.