Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HSPA14 CRISPR/Cas9 KO Plasmid (h): sc-407280
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HSPA14 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HSPA14 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HSPA14 Antibody (G-9): sc-398208
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HSPA14 CRISPR/Cas9 KO Plasmid (h) | sc-407280 | 20 µg | $397.00 |
Overview
HSPA14 (Hsp70-like protein 1) is a member of the HSP70 chaperone family that supports protein quality control by stabilizing nascent polypeptides, promoting correct folding, and limiting aggregation during cellular stress. It participates in proteostasis networks linked to translation, ribosome-associated chaperoning, and stress-responsive pathways that coordinate recovery from heat shock and other proteotoxic insults. By modulating the balance between folding capacity and misfolded-protein handling, HSPA14 helps shape cellular homeostasis in contexts such as oxidative stress, ER–cytosol proteostasis crosstalk, and adaptive stress signaling. Dysregulated chaperone activity and proteostasis imbalance are commonly studied in cancer biology, neurodegeneration, and inflammatory stress models, making HSPA14 a useful target for mechanistic investigations of stress resilience and protein homeostasis.
HSPA14 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the HSPA14 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the HSPA14 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the HSPA14 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HSPA14 protein expression.
This CRISPR knockout system enables efficient generation of HSPA14-deficient cell models for investigation of HSPA14 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting HSPA14 exon(s) critical for HSPA14 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple HSPA14 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HSPA14 CRISPR/Cas9 KO Plasmid (h) and HSPA14 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the HSPA14 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HSPA14 HDR Plasmid (h) and HSPA14 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by HSPA14 homology arms to support homology-directed repair at defined HSPA14 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.