HSP90B1/HSP90 beta Antibody (AT94B9): sc-517405

HSP 90β Antibody (AT94B9) is a mouse monoclonal IgG2b kappa light chain antibody raised against a recombinant protein corresponding to amino acids 22–803 of human HSP90 beta, enabling detection of HSP90 beta of human origin by western blotting (WB), immunofluorescence (IF), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). HSP90 beta is a crucial molecular chaperone involved in proper folding, stabilization, and activation of a wide array of client proteins, which is essential for maintaining cellular homeostasis and facilitating stress responses. HSP90 beta (AT94B9) antibody supports signal transduction pathways, protein degradation, and cell survival mechanisms. Predominantly located in the cytoplasm, HSP90 beta interacts promptly with nascent polypeptides and stress-denatured proteins, underscoring the importance of cellular localization for immediate protective responses. Structurally, HSP90 beta consists of an N-terminal ATPase domain responsible for chaperone activity, a middle domain for client protein interaction, and a C-terminal dimerization domain. These structural features enable ATP-dependent conformational changes necessary for function. Post-translational modifications such as phosphorylation, acetylation, and sumoylation modulate HSP90 beta′s chaperone activity and interactions with co-chaperones and client proteins, thereby influencing cellular signaling pathways and stress responses. These modifications fine-tune function under varying cellular conditions. HSP90 beta interacts with numerous client proteins including kinases like Akt and Raf-1, transcription factors such as p53 and HIF-1α, and steroid hormone receptors, highlighting a central role in regulating processes like cell growth, apoptosis, and response to hypoxia, particularly significant in cancer where these pathways are often dysregulated. In summary, anti-HSP90 beta antibody (AT94B9) provides exceptional specificity and versatility, making this reagent an essential tool for researchers investigating cellular homeostasis, stress responses, and disease-related signaling pathways across various experimental applications.

The preferred HSP90 alpha/beta antibody is HSP90 alpha/beta Antibody (F-8): sc-13119.