HSP 65 Antibody (5177): sc-58170

HSP 65 Antibody (5177) is a mouse monoclonal IgG2a antibody that detects HSP 65 protein of M. tuberculosis origin by western blotting (WB). Anti-HSP 65 antibody (5177) is available as the non-conjugated format, specifically designed to target heat shock protein 65, which plays a crucial role in the survival and pathogenicity of Mycobacterium tuberculosis. This bacterium, a slow-growing obligate aerobic bacillus, causes the majority of tuberculosis cases and possesses a unique thick waxy cell wall that protects against weak disinfectants and enables persistence in dry environments for extended periods. HSP 65 (5177) monoclonal antibody recognizes a protein essential for inducing immune response through interaction with Toll-like receptor 4 (TLR4) to activate NFκB signaling pathways, critical for host immune defense. NFκB activation requires several adaptor proteins, including MyD88, MAL, TRIF, TICAM-2, and MD-2, highlighting the intricate network of interactions that facilitate immune response to Mycobacterium tuberculosis. HSP 65 may influence cytokine expression during infection, underscoring its importance in tuberculosis pathogenesis and making anti-HSP 65 antibody (5177) a valuable tool for studying immune mechanisms involved in this disease.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

HSP 65 Antibody (5177) References:

1. Purification and characterization of three immunodominant proteins (38, 30, and 16 kDa) of Mycobacterium tuberculosis.  |  Devi, KR., et al. 2002. Protein Expr Purif. 24: 188-95. PMID: 11858712
2. Immunoglobulin G, A, and M responses in serum and circulating immune complexes elicited by the 16-kilodalton antigen of Mycobacterium tuberculosis.  |  Raja, A., et al. 2002. Clin Diagn Lab Immunol. 9: 308-12. PMID: 11874868
3. Defining a T-cell epitope within HSP 65 in recurrent aphthous stomatitis.  |  Hasan, A., et al. 2002. Clin Exp Immunol. 128: 318-25. PMID: 11985522
4. Humoral immune response against 38-kDa and 16-kDa mycobacterial antigens in bone and joint tuberculosis.  |  Demkow, U., et al. 2002. Int J Tuberc Lung Dis. 6: 1023-8. PMID: 12475150
5. Autophosphorylation of the 16 kDa and 70 kDa antigens (Hsp 16.3 and Hsp 70) of Mycobacterium tuberculosis.  |  Preneta, R., et al. 2004. Microbiology (Reading). 150: 2135-2141. PMID: 15256556
6. Epitope-specific antibody levels demonstrate recognition of new epitopes and changes in titer but not affinity during treatment of tuberculosis.  |  Bothamley, GH. 2004. Clin Diagn Lab Immunol. 11: 942-51. PMID: 15358657
7. In vitro T-cell immunogenicity of oligopeptides derived from the region 92-110 of the 16-kDa protein of Mycobacterium tuberculosis.  |  Bosze, S., et al. 2004. Biopolymers. 76: 467-76. PMID: 15386261
8. Mycobacterium tuberculosis heat shock proteins use diverse Toll-like receptor pathways to activate pro-inflammatory signals.  |  Bulut, Y., et al. 2005. J Biol Chem. 280: 20961-7. PMID: 15809303
9. Th0 to Th1 switch of CD4 T cell clones specific from the 16-kDa antigen of Mycobacterium tuberculosis after successful therapy: lack of involvement of epitope repertoire and HLA-DR.  |  Caccamo, N., et al. 2005. Immunol Lett. 98: 253-8. PMID: 15860225
10. Antibody profiles characteristic of Mycobacterium tuberculosis infection state.  |  Davidow, A., et al. 2005. Infect Immun. 73: 6846-51. PMID: 16177363
11. Improved laboratory diagnosis of tuberculosis--the Indian experience.  |  Haldar, S., et al. 2011. Tuberculosis (Edinb). 91: 414-26. PMID: 21764383
12. Anti-heat-shock protein antibodies in rheumatic and autoimmune diseases.  |  Tishler, M. and Shoenfeld, Y. 1996. Semin Arthritis Rheum. 26: 558-63. PMID: 8916299

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HSP 65 Antibody (5177) has 6 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HSP 65 Antibody (5177) Specifications

Alternate Product Name: HSP 65

Product Clone Name: 5177

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a

Amino Acids:

Epitope Target: HSP 65

Epitope Species: Mycobacterium tuberculosis

Product Reactivity/Detection: HSP 65

Species Reactivity/Detection: M. tuberculosis

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB

Vialing Information: Each vial contains 100 µg IgG_2a in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for HSP 65 Antibody (5177): N/A