HSP 60 Antibody (B-9): sc-271215

HSP 60 Antibody (B-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects HSP 60 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-HSP 60 antibody (B-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Heat shock protein 60 (HSP 60) plays a crucial role in the mitochondrial matrix, where HSP 60 assists in the proper folding and assembly of newly synthesized proteins, ensuring their functionality and stability. HSP 60′s ability to act as a molecular chaperone is vital for cellular health, as HSP 60 prevents the aggregation of misfolded proteins, which can lead to cellular stress and disease. Additionally, HSP 60 is involved in the immune response, serving as a cell surface marker for γ/δ T cell recognition, thereby linking HSP 60′s chaperone functions to immunological processes. HSP 60′s presence in both the mitochondria and cytosol highlights HSP 60′s importance in maintaining cellular homeostasis and responding to stress, making HSP 60 a significant target for research in various fields, including cancer and neurodegenerative diseases.

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HSP 60 Antibody (B-9) References:

1. Expression of hsp 27, hsp 60, hsc 70, and hsp 70 stress response genes in cultured human urothelial cells (UROtsa) exposed to lethal and sublethal concentrations of sodium arsenite.  |  Rossi, MR., et al. 2002. Environ Health Perspect. 110: 1225-32. PMID: 12460802
2. Identification of a processed protein related to the human chaperonins (hsp 60) protein in mammalian kidney.  |  Ross, WR., et al. 1992. Biochem Biophys Res Commun. 185: 683-7. PMID: 1351721
3. Impairment of vascular function following BCG immunisation is associated with immune responses to HSP-60 in the cholesterol-fed rabbit.  |  Lamb, DJ., et al. 2004. Atherosclerosis. 172: 13-20. PMID: 14709352
4. Significance of serum antibodies against HSP 60 and HSP 70 for the diagnostic of infectious diseases.  |  Bleotu, C., et al. 2014. Virulence. 5: 828-31. PMID: 25483863
5. The effects of icariin on the expression of HIF-1α, HSP-60 and HSP-70 in PC12 cells suffered from oxygen-glucose deprivation-induced injury.  |  Mo, ZT., et al. 2017. Pharm Biol. 55: 848-852. PMID: 28140748
6. Mitochondrial chaperone HSP-60 regulates anti-bacterial immunity via p38 MAP kinase signaling.  |  Jeong, DE., et al. 2017. EMBO J. 36: 1046-1065. PMID: 28283579
7. Heat shock proteins with an emphasis on HSP 60.  |  Malik, JA. and Lone, R. 2021. Mol Biol Rep. 48: 6959-6969. PMID: 34498161
8. Protein folding in the cell: functions of two families of molecular chaperone, hsp 60 and TF55-TCP1.  |  Horwich, AL. and Willison, KR. 1993. Philos Trans R Soc Lond B Biol Sci. 339: 313-25; discussion 325-6. PMID: 8098536
9. Major cytoplasmic membrane protein of Legionella pneumophila, a genus common antigen and member of the hsp 60 family of heat shock proteins, induces protective immunity in a guinea pig model of Legionnaires' disease.  |  Blander, SJ. and Horwitz, MA. 1993. J Clin Invest. 91: 717-23. PMID: 8432872
10. Experimental immunization with anti-rheumatic bacterial extract OM-89 induces T cell responses to heat shock protein (hsp)60 and hsp70; modulation of peripheral immunological tolerance as its possible mode of action in the treatment of rheumatoid arthritis (RA).  |  Bloemendal, A., et al. 1997. Clin Exp Immunol. 110: 72-8. PMID: 9353151

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HSP 60 Antibody (B-9) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-HSP 60 Antibody (B-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HSP 60 Antibody (B-9) Specifications

Alternate Product Name: HSP 60

Product Clone Name: B-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 274-573

Epitope Target: HSP 60

Epitope Species: human

Product Reactivity/Detection: HSP 60

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for HSP 60 Antibody (B-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred HSP 60 antibody is HSP 60 Antibody (H-1): sc-13115.