Date published: 2025-12-14

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HSP 60 Antibody (B-9): sc-271215

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Datasheets
  • HSP 60 Antibody (B-9) is a mouse monoclonal IgG1 κ, cited in 13 publications, provided at 200 µg/ml
  • raised against amino acids 274-573 of HSP 60 of human origin
  • Anti-HSP 60 Antibody (B-9) is recommended for detection of HSP 60 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
  • Anti-HSP 60 Antibody (B-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • m-IgG1 BP-HRP is the preferred secondary detection reagent for HSP 60 Antibody (B-9) for WB and IHC(P) applications. This reagent is now offered in a bundle with HSP 60 Antibody (B-9) (see ordering information below).

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HSP 60 Antibody (B-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects HSP 60 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-HSP 60 antibody (B-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Heat shock protein 60 (HSP 60) plays a crucial role in the mitochondrial matrix, where HSP 60 assists in the proper folding and assembly of newly synthesized proteins, ensuring their functionality and stability. HSP 60′s ability to act as a molecular chaperone is vital for cellular health, as HSP 60 prevents the aggregation of misfolded proteins, which can lead to cellular stress and disease. Additionally, HSP 60 is involved in the immune response, serving as a cell surface marker for γ/δ T cell recognition, thereby linking HSP 60′s chaperone functions to immunological processes. HSP 60′s presence in both the mitochondria and cytosol highlights HSP 60′s importance in maintaining cellular homeostasis and responding to stress, making HSP 60 a significant target for research in various fields, including cancer and neurodegenerative diseases.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

HSP 60 Antibody (B-9)

sc-271215
200 µg/ml
$316.00

HSP 60 Antibody (B-9): m-IgG1 BP-HRP Bundle

sc-531684
200 µg Ab; 20 µg BP
$354.00

HSP 60 Antibody (B-9) AC

sc-271215 AC
500 µg/ml, 25% agarose
$416.00

HSP 60 Antibody (B-9) HRP

sc-271215 HRP
200 µg/ml
$316.00

HSP 60 Antibody (B-9) FITC

sc-271215 FITC
200 µg/ml
$330.00

HSP 60 Antibody (B-9) PE

sc-271215 PE
200 µg/ml
$343.00

HSP 60 Antibody (B-9) Alexa Fluor® 488

sc-271215 AF488
200 µg/ml
$357.00

HSP 60 Antibody (B-9) Alexa Fluor® 546

sc-271215 AF546
200 µg/ml
$357.00

HSP 60 Antibody (B-9) Alexa Fluor® 594

sc-271215 AF594
200 µg/ml
$357.00

HSP 60 Antibody (B-9) Alexa Fluor® 647

sc-271215 AF647
200 µg/ml
$357.00

HSP 60 Antibody (B-9) Alexa Fluor® 680

sc-271215 AF680
200 µg/ml
$357.00

HSP 60 Antibody (B-9) Alexa Fluor® 790

sc-271215 AF790
200 µg/ml
$357.00

I am using HSP 60 (B-9): sc-271215 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jenniferc
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Perfect single bandsWe are very satisfied with this antibody. Worked as expected and we recommend this antibody.
Date published: 2018-04-13
Rated 5 out of 5 by from Excellent band for WesternGave strong bands at expected size without significant background
Date published: 2017-06-29
Rated 5 out of 5 by from Very Versatile AntibodyThis antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC and ELISA. Strong, clear signal with little to no background. Beautiful tissue staining. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-01-24
Rated 5 out of 5 by from Works well for IF applicationsWorks well for IF applications, mitochondrial localization in MeOH fixed HeLa cells. -SCBT QC
Date published: 2015-04-15
Rated 5 out of 5 by from Works great for IFWorks great for IF!
Date published: 2015-03-17
Rated 5 out of 5 by from Robust staining in paraffinRobust staining in paraffin-embedded human rectum. -SCBT QC
Date published: 2015-02-24
Rated 5 out of 5 by from Works great for Western blotWorks great for Western blot, single strong bands in HeLa and KNRK. -SCBT QC
Date published: 2015-02-22
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HSP 60 Antibody (B-9) is rated 5.0 out of 5 by 8.
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