HSP 60 Antibody (H-1): sc-13115

HSP 60 Antibody (H-1) is a mouse monoclonal IgG3 kappa light chain antibody that detects HSP 60 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-HSP 60 antibody (H-1) is available in a non-conjugated form, allowing for versatile experimental use. HSP 60, a key member of the heat shock protein family, plays a crucial role in mitochondrial function by assisting in the proper folding and assembly of newly synthesized proteins destined for the mitochondrial matrix. This process maintains cellular homeostasis and ensures proteins achieve functional conformations, essential for cellular metabolism and energy production. HSP 60 also participates in immune response, acting as a cell surface marker that facilitates recognition of stressed or damaged cells by γ/δ T cells, linking cellular stress responses to immune surveillance. HSP 60′s dual function within mitochondria and on cell surfaces highlights its importance in both cellular function and immune regulation, making HSP 60 monoclonal antibody (H-1) a valuable research tool for studying these critical biological processes.

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HSP 60 Antibody (H-1) References:

1. Expression of hsp 27, hsp 60, hsc 70, and hsp 70 stress response genes in cultured human urothelial cells (UROtsa) exposed to lethal and sublethal concentrations of sodium arsenite.  |  Rossi, MR., et al. 2002. Environ Health Perspect. 110: 1225-32. PMID: 12460802
2. Antifolding activity of hsp60 couples protein import into the mitochondrial matrix with export to the intermembrane space.  |  Koll, H., et al. 1992. Cell. 68: 1163-75. PMID: 1347713
3. Prevention of protein denaturation under heat stress by the chaperonin Hsp60.  |  Martin, J., et al. 1992. Science. 258: 995-8. PMID: 1359644
4. Heat shock protein Hsp60-reactive gamma delta cells: a large, diversified T-lymphocyte subset with highly focused specificity.  |  O'Brien, RL., et al. 1992. Proc Natl Acad Sci U S A. 89: 4348-52. PMID: 1584768
5. Significance of serum antibodies against HSP 60 and HSP 70 for the diagnostic of infectious diseases.  |  Bleotu, C., et al. 2014. Virulence. 5: 828-31. PMID: 25483863
6. The effects of icariin on the expression of HIF-1α, HSP-60 and HSP-70 in PC12 cells suffered from oxygen-glucose deprivation-induced injury.  |  Mo, ZT., et al. 2017. Pharm Biol. 55: 848-852. PMID: 28140748
7. Mitochondrial chaperone HSP-60 regulates anti-bacterial immunity via p38 MAP kinase signaling.  |  Jeong, DE., et al. 2017. EMBO J. 36: 1046-1065. PMID: 28283579
8. Heat shock proteins with an emphasis on HSP 60.  |  Malik, JA. and Lone, R. 2021. Mol Biol Rep. 48: 6959-6969. PMID: 34498161
9. Transient rates of synthesis of individual polypeptides in E. coli following temperature shifts.  |  Lemaux, PG., et al. 1978. Cell. 13: 427-34. PMID: 350413
10. The effect of amino acid analogues and heat shock on gene expression in chicken embryo fibroblasts.  |  Kelley, PM. and Schlesinger, MJ. 1978. Cell. 15: 1277-86. PMID: 569556
11. Human peripheral gamma delta T cells recognize hsp60 molecules on Daudi Burkitt's lymphoma cells.  |  Kaur, I., et al. 1993. J Immunol. 150: 2046-55. PMID: 8094731
12. Genetic complexity of the human hsp 60 gene.  |  Pochon, NA. and Mach, B. 1996. Int Immunol. 8: 221-30. PMID: 8671607

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HSP 60 Antibody (H-1) has 162 citations in a variety of scientific publications.

Primary antibodies like Anti-HSP 60 Antibody (H-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HSP 60 Antibody (H-1) Specifications

Alternate Product Name: HSP 60

Product Clone Name: H-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 31-50

Epitope Target: HSP 60

Epitope Species: human

Product Reactivity/Detection: HSP 60

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 162 citations in various scientific publications

Available Conjugates/Forms for HSP 60 Antibody (H-1): P: Blocking Peptide