Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HSP 40 Antibody (B-3): sc-398766. Western blot analysis of HSP 40 expression in untreated HeLa (A), chemically-treated HeLa (B, C), untreated K-562 (D) and chemically-treated K-562 (E, F) whole cell lysates. β-Actin (C4): sc-47778 used as loading control. Detection reagent used: m-IgG Fc BP-HRP: sc-525409.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HSP 40 CRISPR/Cas9 KO Plasmid (h): sc-402810
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HSP 40 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HSP 40 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HSP 40 Antibody (B-3): sc-398766
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HSP 40 CRISPR/Cas9 KO Plasmid (h) | sc-402810 | 20 µg | $397.00 |