HSP 27 Antibody (HSP25-31) recommended for detection of HSP 27 of mouse, rat, human, porcine, feline and canine origin by WB, IP and IF

HSP 27 Antibody (HSP25-31): sc-51958

HSP 27 Antibody (HSP25-31) is rated 3.3 out of 5 by 3.
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Datasheets

    • HSP 27 Antibody (HSP25-31) is a mouse monoclonal IgG2b provided at 100 µg/ml
    • raised against recombinant HSP 27 of chicken origin
    • recommended for detection of HSP 27 of mouse, rat, human, porcine, feline and canine origin by WB, IP and IF
    • See HSP 27 (F-4): sc-13132 for HSP 27 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

For Western Blot, is it recommended to use denatured or non-denatured conditions with HSP 27 (HSP25-31): sc-51958 antibody?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 3 out of 5 by from Antibody has mixed results in human cell lines Antibody has mixed results in human cell lines, but detection appears specific. -SCBT QC
Date published: 2015-03-13
Rated 3 out of 5 by from Able to detect antigen in transfected cells Able to detect antigen in transfected cells, but did not detect endogenous protein in human cell lysate. -SCBT QC
Date published: 2015-03-01
Rated 4 out of 5 by from Shows strong recognition of protein in HeLa cells Shows strong recognition of protein in HeLa cells. -SCBT QC
Date published: 2015-01-26
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