HSPA8/HSC70 Antibody (B-6): sc-7298

HSC 70 Antibody (B-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects HSC 70 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). HSC 70 (B-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HSC 70, also known as Heat Shock Cognate 70, plays a crucial role in cellular stress responses and protein homeostasis. HSC 70 functions primarily as a molecular chaperone, assisting in proper folding of nascent polypeptide chains and preventing aggregation of misfolded proteins, which is vital for maintaining cellular integrity and function. HSC 70 is predominantly located in cytosol and nucleus of mammalian cells, where HSC 70 interacts with various client proteins, ensuring correct folding and assembly into functional complexes. This localization allows rapid response to cellular stressors, such as heat shock or oxidative stress, thereby protecting cells from damage and promoting survival. HSC 70′s ability to facilitate protein translocation across membranes further underscores significance in cellular processes, including protein import into mitochondria and endoplasmic reticulum. Using anti-HSC 70 antibody (B-6) in research can provide valuable insights into mechanisms of protein folding and cellular stress response, contributing to deeper understanding of various diseases associated with protein misfolding and aggregation.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

HSPA8/HSC70 Antibody (B-6) References:

1. Prevention of protein denaturation under heat stress by the chaperonin Hsp60.  |  Martin, J., et al. 1992. Science. 258: 995-8. PMID: 1359644
2. Effects of low culture temperature on the induction of hsp70 mRNA and the accumulation of hsp70 and hsp105 in mouse FM3A cells.  |  Hatayama, T., et al. 1992. J Biochem. 111: 484-90. PMID: 1618738
3. The stress protein heat shock cognate 70 (Hsc70) inhibits the Transient Receptor Potential Vanilloid type 1 (TRPV1) channel.  |  Iftinca, M., et al. 2016. Mol Pain. 12: PMID: 27558883
4. Heat Shock Cognate 70 Inhibitor, VER-155008, Reduces Memory Deficits and Axonal Degeneration in a Mouse Model of Alzheimer's Disease.  |  Yang, X. and Tohda, C. 2018. Front Pharmacol. 9: 48. PMID: 29441022
5. Heat shock cognate 70 genes contribute to Drosophila spermatocyte growth progression possibly through the insulin signaling pathway.  |  Azuma, M., et al. 2021. Dev Growth Differ. 63: 231-248. PMID: 34050930
6. A plastid-targeted heat shock cognate 70-kDa protein confers osmotic stress tolerance by enhancing ROS scavenging capability.  |  Ding, F., et al. 2022. Front Plant Sci. 13: 1012145. PMID: 36275553
7. Heat shock cognate 70 protein like-2 protein in camphor pollen is one of the major culprits of asthma.  |  Cao, Y., et al. 2023. Mol Immunol. 156: 170-176. PMID: 36933345
8. Cloning of rat grp75, an hsp70-family member, and its expression in normal and ischemic brain.  |  Massa, SM., et al. 1995. J Neurosci Res. 40: 807-19. PMID: 7629893
9. Protein-mediated protein maturation in eukaryotes.  |  Haas, IG. 1995. FEBS Lett. 369: 72-5. PMID: 7641888
10. Can Hsp70 proteins act as force-generating motors?  |  Glick, BS. 1995. Cell. 80: 11-4. PMID: 7813006
11. Cloning and subcellular localization of human mitochondrial hsp70.  |  Bhattacharyya, T., et al. 1995. J Biol Chem. 270: 1705-10. PMID: 7829505
12. Autophosphorylation of grp94 (endoplasmin).  |  Csermely, P., et al. 1995. J Biol Chem. 270: 6381-8. PMID: 7890776

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HSPA8/HSC70 Antibody (B-6) has 878 citations in a variety of scientific publications.

Primary antibodies like Anti-HSPA8/HSC70 Antibody (B-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HSPA8/HSC70 Antibody (B-6) Specifications

Alternate Product Name: HSC70

Product Clone Name: B-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 583-601

Epitope Target: HSC 70

Epitope Species: human

Product Reactivity/Detection: HSC 70

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 878 citations in various scientific publications

Available Conjugates/Forms for HSPA8/HSC70 Antibody (B-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred HSC70/HSP70 antibody is HSC70/HSP70 Antibody (W27): sc-24.