Hrs Antibody (C-7): sc-271455

Hrs Antibody (C-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects Hrs protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Hrs antibody (C-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs) is a crucial zinc-finger protein that plays a significant role in intracellular trafficking and signal transduction, particularly associated with early endosomes. Hrs is known to interact with signaling proteins such as STAM and undergoes tyrosine phosphorylation in response to cytokines like interleukin-2, granulocyte-macrophage colony-stimulating factor, or hepatocyte growth factor. Hrs structure includes a phosphatidylinositol 3-phosphate-binding domain, which is vital for endosomal targeting, allowing Hrs to co-localize with Clathrin through a Clathrin box motif located at its carboxy-terminus. This interaction is essential for the proper functioning of endosomal sorting processes. Additionally, Hrs is integral to ventral folding morphogenesis and shares structural similarities with the yeast protein Vps27p, which is involved in vacuolar protein sorting. The human Hrs gene, located on chromosome 17q25, encodes a 777 amino acid protein, and in Schwann cells, Hrs co-localizes with the tumor suppressor protein schwannomin at endosomes, indicating a potential role for schwannomin in Hrs-mediated cell signaling pathways.

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Hrs Antibody (C-7) References:

1. The neurofibromatosis 2 tumor suppressor protein interacts with hepatocyte growth factor-regulated tyrosine kinase substrate.  |  Scoles, DR., et al. 2000. Hum Mol Genet. 9: 1567-74. PMID: 10861283
2. Function of Hrs in endocytic trafficking and signalling.  |  Raiborg, C., et al. 2001. Biochem Soc Trans. 29: 472-5. PMID: 11498011
3. Hrs recruits clathrin to early endosomes.  |  Raiborg, C., et al. 2001. EMBO J. 20: 5008-21. PMID: 11532964
4. Exosome secretion of dendritic cells is regulated by Hrs, an ESCRT-0 protein.  |  Tamai, K., et al. 2010. Biochem Biophys Res Commun. 399: 384-90. PMID: 20673754
5. Recycling of EGFR and ErbB2 is associated with impaired Hrs tyrosine phosphorylation and decreased deubiquitination by AMSH.  |  Meijer, IM., et al. 2012. Cell Signal. 24: 1981-8. PMID: 22800866
6. Hrs- and CD63-dependent competing mechanisms make different sized endosomal intraluminal vesicles.  |  Edgar, JR., et al. 2014. Traffic. 15: 197-211. PMID: 24279430
7. Ubpy controls the stability of the ESCRT-0 subunit Hrs in development.  |  Zhang, J., et al. 2014. Development. 141: 1473-9. PMID: 24574010
8. HRS-WASH axis governs actin-mediated endosomal recycling and cell invasion.  |  MacDonald, E., et al. 2018. J Cell Biol. 217: 2549-2564. PMID: 29891722
9. Syk and Hrs Regulate TLR3-Mediated Antiviral Response in Murine Astrocytes.  |  Mielcarska, MB., et al. 2019. Oxid Med Cell Longev. 2019: 6927380. PMID: 31089414
10. The ESCRT-0 Protein HRS Interacts with the Human T Cell Leukemia Virus Type 2 Antisense Protein APH-2 and Suppresses Viral Replication.  |  Martini, F., et al. 2019. J Virol. 94: PMID: 31597781
11. Hrs is associated with STAM, a signal-transducing adaptor molecule. Its suppressive effect on cytokine-induced cell growth.  |  Asao, H., et al. 1997. J Biol Chem. 272: 32785-91. PMID: 9407053
12. Human Hrs, a tyrosine kinase substrate in growth factor-stimulated cells: cDNA cloning and mapping of the gene to chromosome 17.  |  Lu, L., et al. 1998. Gene. 213: 125-32. PMID: 9630564

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Hrs Antibody (C-7) has 16 citations in a variety of scientific publications.

Primary antibodies like Anti-Hrs Antibody (C-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Hrs Antibody (C-7) Specifications

Alternate Product Name: HGS

Product Clone Name: C-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 121-173

Epitope Target: Hrs

Epitope Species: human

Product Reactivity/Detection: Hrs

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 16 citations in various scientific publications

Available Conjugates/Forms for Hrs Antibody (C-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin