HR Antibody (A-11): sc-514686

HR Antibody (A-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects HR protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-HR antibody (A-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HR protein, also known as Hairless, is a 1,189 amino acid protein that exists in two isoforms due to alternative splicing, with isoform 1 being more prevalent than isoform 2. These isoforms are expressed in various tissues at different concentrations; for instance, isoform 1 is found at low levels in the kidneys and testis, while isoform 2 is predominantly expressed in the skin. Both isoforms are co-expressed in several tissues, with strong expression in the small intestine and brain, and weak expression in the trachea. HR plays a crucial role as a transcription factor in the regulation of hair growth, which occurs in three distinct phases: anagen (growth), catagen (regression), and telogen (rest). Although the exact mechanisms remain unclear, HR influences one of these hair growth phases and collaborates with the vitamin D receptor to regulate hair follicle cycling. Deficiencies in HR can lead to severe conditions such as alopecia universalis congenita and atrichia with papular lesions, both of which are autosomal recessive disorders. Alopecia universalis congenita is characterized by the absence of hair follicles, while atrichia with papular lesions presents with papular lesions covering the body and minimal to no hair growth.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

HR Antibody (A-11) References:

1. The hairless gene mutated in congenital hair loss disorders encodes a novel nuclear receptor corepressor.  |  Potter, GB., et al. 2001. Genes Dev. 15: 2687-701. PMID: 11641275
2. Ligand-independent actions of the vitamin D receptor maintain hair follicle homeostasis.  |  Skorija, K., et al. 2005. Mol Endocrinol. 19: 855-62. PMID: 15591533
3. Hairless contains a novel nuclear matrix targeting signal and associates with histone deacetylase 3 in nuclear speckles.  |  Djabali, K. and Christiano, AM. 2004. Differentiation. 72: 410-8. PMID: 15606500
4. The alopecias associated with vitamin D-dependent rickets type IIA and with hairless gene mutations: a comparative clinical, histologic, and immunohistochemical study.  |  Bergman, R., et al. 2005. Arch Dermatol. 141: 343-51. PMID: 15781675
5. [Molecular cloning of full-long cDNA sequences encoding hairless gene in the Kunming mouse].  |  Zhang, JT., et al. 2005. Yi Chuan. 27: 908-14. PMID: 16378938
6. Development and progression of alopecia in the vitamin D receptor null mouse.  |  Bikle, DD., et al. 2006. J Cell Physiol. 207: 340-53. PMID: 16419036
7. The 'bald Mill Hill' mutation in the mouse is associated with an abnormal, mislocalized HR bmh protein.  |  Brancaz-Bouvier, MV., et al. 2008. J Invest Dermatol. 128: 311-21. PMID: 17657241
8. Ligand-independent regulation of the hairless promoter by vitamin D receptor.  |  Engelhard, A., et al. 2008. Photochem Photobiol. 84: 515-21. PMID: 18266815

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HR Antibody (A-11) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-HR Antibody (A-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HR Antibody (A-11) Specifications

Alternate Product Name: HR

Product Clone Name: A-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-300

Epitope Target: HR

Epitope Species: mouse

Product Reactivity/Detection: HR

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for HR Antibody (A-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin