HPV16 L1 Antibody (CAMVIR-1): sc-47699

HPV16 L1 Antibody (CAMVIR-1) is a mouse monoclonal IgG2a kappa light chain antibody that detects HPV16 L1 protein of HPV-16 origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). Anti-HPV16 L1 antibody (CAMVIR-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HPV16 L1 protein plays a crucial role in the viral life cycle, forming the major structural component of the virus′s capsid, which is essential for protecting and delivering viral genome into host cells. Pentameric assembly of L1 proteins creates a stable shell that facilitates initial attachment to host cell surface, a critical step in the infection process. C-terminus of L1 participates in both DNA binding and packaging, underscoring importance in viral replication cycle. Expression of L1 protein remains restricted to upper layers of infected epithelium, significant for understanding pathogenesis of HPV-related diseases, including cervical cancer. HPV16 L1′s ability to package unrelated plasmid DNA in vitro and deliver to eukaryotic cells highlights potential for gene therapy applications. Distinct localization patterns of L1 and interaction with minor capsid protein L2, found in punctate nuclear regions known as promonocytic leukemia protein oncogenic domains (PODs), provide insights into complex dynamics of HPV infection and cellular mechanisms involved.

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HPV16 L1 Antibody (CAMVIR-1) References:

1. Nasal immunization of mice with human papillomavirus type 16 (HPV-16) virus-like particles or with the HPV-16 L1 gene elicits specific cytotoxic T lymphocytes in vaginal draining lymph nodes.  |  Dupuy, C., et al. 1999. J Virol. 73: 9063-71. PMID: 10516012
2. The human papillomavirus type 16 negative regulatory RNA element interacts with three proteins that act at different posttranscriptional levels.  |  Koffa, MD., et al. 2000. Proc Natl Acad Sci U S A. 97: 4677-82. PMID: 10781073
3. Structure of small virus-like particles assembled from the L1 protein of human papillomavirus 16.  |  Chen, XS., et al. 2000. Mol Cell. 5: 557-67. PMID: 10882140
4. The nine C-terminal amino acids of the major capsid protein of the human papillomavirus type 16 are essential for DNA binding and gene transfer capacity.  |  Touzé, A., et al. 2000. FEMS Microbiol Lett. 189: 121-7. PMID: 10913877
5. Mucosal vaccination with a recombinant Salmonella typhimurium expressing human papillomavirus type 16 (HPV16) L1 virus-like particles (VLPs) or HPV16 VLPs purified from insect cells inhibits the growth of HPV16-expressing tumor cells in mice.  |  Revaz, V., et al. 2001. Virology. 279: 354-60. PMID: 11145916
6. Human papillomavirus type 16 minor capsid protein l2 N-terminal region containing a common neutralization epitope binds to the cell surface and enters the cytoplasm.  |  Kawana, Y., et al. 2001. J Virol. 75: 2331-6. PMID: 11160736
7. L1 interaction domains of papillomavirus l2 necessary for viral genome encapsidation.  |  Okun, MM., et al. 2001. J Virol. 75: 4332-42. PMID: 11287582
8. Vaccine regimen for prevention of sexually transmitted infections with human papillomavirus type 16.  |  Kowalczyk, DW., et al. 2001. Vaccine. 19: 3583-90. PMID: 11348726
9. HPV16 L1 diversity and its potential impact on the vaccination-induced immunity.  |  El Aliani, A., et al. 2020. Gene. 747: 144682. PMID: 32304786
10. Mutation Profile of HPV16 L1 and L2 Genes in Different Geographic Areas.  |  Tsakogiannis, D., et al. 2022. Viruses. 15: PMID: 36680181
11. The papillomavirus minor capsid protein, L2, induces localization of the major capsid protein, L1, and the viral transcription/replication protein, E2, to PML oncogenic domains.  |  Day, PM., et al. 1998. J Virol. 72: 142-50. PMID: 9420209
12. In vitro infection and type-restricted antibody-mediated neutralization of authentic human papillomavirus type 16.  |  White, WI., et al. 1998. J Virol. 72: 959-64. PMID: 9444988

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-HPV16 L1 Antibody (CAMVIR-1) has 19 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

HPV16 L1 Antibody (CAMVIR-1) Specifications

Alternate Product Name: HPV16 L1

Product Clone Name: CAMVIR-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 198-531

Epitope Target: HPV16 L1

Epitope Species: viral

Product Reactivity/Detection: HPV16 L1; may cross react with HPV33

Species Reactivity/Detection: HPV-16

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 19 citations in various scientific publications

Available Conjugates/Forms for HPV16 L1 Antibody (CAMVIR-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin