HPV16 E2 Antibody (TVG 271): sc-53327
- HPV16 E2 Antibody (TVG 271) is a mouse monoclonal IgG1 κ HPV16 E2 antibody, cited in 3 publications, provided at 200 µg/ml
- raised against vaccinia-E2 followed by intravenous injection of maltose binding protein MBP-E2
- HPV16 E2 Antibody (TVG 271) is recommended for detection of HPV16 E2 by WB
- Anti-HPV16 E2 Antibody (TVG 271) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for HPV16 E2 Antibody (TVG 271) for WB applications. These reagents are now offered in bundles with HPV16 E2 Antibody (TVG 271) (see ordering information below).
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HPV16 E2 Antibody (TVG 271) is a mouse monoclonal IgG1 kappa light chain antibody that detects HPV16 E2 protein of HPV-16 E2 origin by western blotting (WB). Anti-HPV16 E2 antibody (TVG 271) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The human papillomavirus (HPV) family includes HPV16, a strain significantly associated with cervical cancer cases. HPV16 E2 protein plays a crucial role in regulating viral DNA replication and maintaining infected cell homeostasis. Specifically, HPV16 E2 is essential for transcriptional regulation of E6 and E7 oncoproteins, binding to four sites within the viral long control region and potentially interacting with nuclear hormone receptors. This regulation is vital because overexpression of E6 and E7, often resulting from HPV16 E2 gene disruption during HPV genome integration into host DNA, can lead to malignant transformation in cervical cancer. HPV16 E2 has been shown to induce apoptotic cell death through two distinct pathways: one involving binding of tumor suppressor protein p53 and another through direct interaction with the viral genome, highlighting importance in both viral pathogenesis and potential therapeutic strategies.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
HPV16 E2 Antibody (TVG 271) References:
- A deletion in the gene encoding the CD45 antigen in a patient with SCID. | Tchilian, EZ., et al. 2001. J Immunol. 166: 1308-13. PMID: 11145714
- Human papillomavirus type 16 E2 and E6/E7 variants. | Swan, DC., et al. 2005. Gynecol Oncol. 96: 695-700. PMID: 15721413
- HPV16 E2 gene disruption and polymorphisms of E2 and LCR: some significant associations with cervical cancer in Indian women. | Bhattacharjee, B. and Sengupta, S. 2006. Gynecol Oncol. 100: 372-8. PMID: 16246404
- The HPV16 E2 transcriptional regulator mode of action depends on the physical state of the viral genome. | Schmidt, MT., et al. 2005. Acta Biochim Pol. 52: 823-32. PMID: 16273126
- Combined prophylactic and therapeutic cancer vaccine: enhancing CTL responses to HPV16 E2 using a chimeric VLP in HLA-A2 mice. | Qian, J., et al. 2006. Int J Cancer. 118: 3022-9. PMID: 16425257
- Repression of HPV16 early region transcription by the E2 protein. | Soeda, E., et al. 2006. Virology. 351: 29-41. PMID: 16624362
- Structural and thermodynamic basis for the enhanced transcriptional control by the human papillomavirus strain-16 E2 protein. | Cicero, DO., et al. 2006. Biochemistry. 45: 6551-60. PMID: 16716065
- Orphan nuclear hormone receptor NR4A1 interacts with HPV16 E2 regulatory protein. | Olejnik-Schmidt, AK., et al. 2006. Cell Mol Biol Lett. 11: 102-8. PMID: 16847753
- The interaction between human papillomavirus type 16 E1 and E2 proteins is blocked by an antibody to the N-terminal region of E2. | Hibma, MH., et al. 1995. Eur J Biochem. 229: 517-25. PMID: 7744075
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HPV16 E2 Antibody (TVG 271) | sc-53327 | 200 µg/ml | $322.00 | |||
HPV16 E2 Antibody (TVG 271): m-IgG Fc BP-HRP Bundle | sc-528562 | 200 µg Ab; 10 µg BP | $361.00 | |||
HPV16 E2 Antibody (TVG 271): m-IgGκ BP-HRP Bundle | sc-520968 | 200 µg Ab, 40 µg BP | $361.00 | |||
HPV16 E2 Antibody (TVG 271): m-IgG1 BP-HRP Bundle | sc-542997 | 200 µg Ab; 20 µg BP | $361.00 | |||
HPV16 E2 Antibody (TVG 271) AC | sc-53327 AC | 500 µg/ml, 25% agarose | $424.00 | |||
HPV16 E2 Antibody (TVG 271) HRP | sc-53327 HRP | 200 µg/ml | $322.00 | |||
HPV16 E2 Antibody (TVG 271) FITC | sc-53327 FITC | 200 µg/ml | $336.00 | |||
HPV16 E2 Antibody (TVG 271) PE | sc-53327 PE | 200 µg/ml | $349.00 | |||
HPV16 E2 Antibody (TVG 271) Alexa Fluor® 488 | sc-53327 AF488 | 200 µg/ml | $364.00 | |||
HPV16 E2 Antibody (TVG 271) Alexa Fluor® 546 | sc-53327 AF546 | 200 µg/ml | $364.00 | |||
HPV16 E2 Antibody (TVG 271) Alexa Fluor® 594 | sc-53327 AF594 | 200 µg/ml | $364.00 | |||
HPV16 E2 Antibody (TVG 271) Alexa Fluor® 647 | sc-53327 AF647 | 200 µg/ml | $364.00 | |||
HPV16 E2 Antibody (TVG 271) Alexa Fluor® 680 | sc-53327 AF680 | 200 µg/ml | $364.00 | |||
HPV16 E2 Antibody (TVG 271) Alexa Fluor® 790 | sc-53327 AF790 | 200 µg/ml | $364.00 |