Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HPS-4 CRISPR/Cas9 KO Plasmid (h): sc-404822
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HPS-4 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HPS-4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HPS-4 Antibody (A-6): sc-398070
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HPS-4 CRISPR/Cas9 KO Plasmid (h) | sc-404822 | 20 µg | $397.00 |
Overview
HPS4 encodes HPS-4, a core component of the biogenesis of lysosome-related organelles complex-3 (BLOC-3), which functions as a guanine nucleotide exchange factor regulating Rab32/Rab38-dependent membrane trafficking. Through these pathways, HPS-4 supports cargo sorting and delivery to lysosomes and lysosome-related organelles, influencing melanosome maturation, platelet dense granule formation, and endolysosomal homeostasis. Disruption of HPS4 is linked to Hermansky–Pudlak syndrome type 4, a disorder characterized by defects in organelle biogenesis and vesicular transport. HPS4 is therefore widely used to probe endosomal dynamics, organelle maturation, and cellular phenotypes associated with impaired lysosomal trafficking.
HPS-4 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the HPS4 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the HPS4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the HPS4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HPS-4 protein expression.
This CRISPR knockout system enables efficient generation of HPS4-deficient cell models for investigation of HPS-4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting HPS4 exon(s) critical for HPS-4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple HPS4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HPS-4 CRISPR/Cas9 KO Plasmid (h) and HPS-4 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the HPS4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HPS-4 HDR Plasmid (h) and HPS-4 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by HPS4 homology arms to support homology-directed repair at defined HPS4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.