HP1β Antibody (MAC353): sc-56704

HP1β Antibody (MAC353) is a rat monoclonal IgG2b antibody that detects HP1β in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). HP1β (MAC353) antibody is available in a non-conjugated form, allowing for versatile experimental use. HP1β, or heterochromatin protein 1 beta, plays a crucial role in the organization and maintenance of heterochromatin, which is essential for regulating gene expression and ensuring genomic stability. HP1β is primarily located in the nucleus, where HP1β binds to methylated histones and contributes to the formation of compact chromatin structures that are transcriptionally inactive. HP1β presence in heterochromatin is vital for the silencing of repetitive DNA elements and the prevention of genomic instability, which can lead to various diseases, including cancer. Additionally, HP1β interacts with other proteins involved in chromatin remodeling and DNA repair, further underscoring HP1β importance in cellular processes. Anti-HP1β antibody (MAC353) enables researchers to gain insights into the dynamics of chromatin organization and the functional implications of HP1β in various biological contexts.

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HP1β Antibody (MAC353) References:

1. Localization and phosphorylation of HP1 proteins during the cell cycle in mammalian cells.  |  Minc, E., et al. 1999. Chromosoma. 108: 220-34. PMID: 10460410
2. Heterochromatin dynamics in mouse cells: interaction between chromatin assembly factor 1 and HP1 proteins.  |  Murzina, N., et al. 1999. Mol Cell. 4: 529-40. PMID: 10549285
3. Duplication and maintenance of heterochromatin domains.  |  Taddei, A., et al. 1999. J Cell Biol. 147: 1153-66. PMID: 10601331
4. Identification of heterochromatin protein 1 (HP1) as a phosphorylation target by Pim-1 kinase and the effect of phosphorylation on the transcriptional repression function of HP1(1).  |  Koike, N., et al. 2000. FEBS Lett. 467: 17-21. PMID: 10664448
5. Purification and characterization of CAF-I, a human cell factor required for chromatin assembly during DNA replication in vitro.  |  Smith, S. and Stillman, B. 1989. Cell. 58: 15-25. PMID: 2546672
6. Dynamic and flexible H3K9me3 bridging via HP1β dimerization establishes a plastic state of condensed chromatin.  |  Hiragami-Hamada, K., et al. 2016. Nat Commun. 7: 11310. PMID: 27090491
7. Critical role for TRIM28 and HP1β/γ in the epigenetic control of T cell metabolic reprograming and effector differentiation.  |  Gehrmann, U., et al. 2019. Proc Natl Acad Sci U S A. 116: 25839-25849. PMID: 31776254
8. Role of HP1β during spermatogenesis and DNA replication.  |  Charaka, V., et al. 2020. Chromosoma. 129: 215-226. PMID: 32651609
9. HP1β carries an acidic linker domain and requires H3K9me3 for phase separation.  |  Qin, W., et al. 2021. Nucleus. 12: 44-57. PMID: 33660589
10. Phosphorylation of the HP1β hinge region sequesters KAP1 in heterochromatin and promotes the exit from naïve pluripotency.  |  Qin, W., et al. 2021. Nucleic Acids Res. 49: 7406-7423. PMID: 34214177
11. The p150 and p60 subunits of chromatin assembly factor I: a molecular link between newly synthesized histones and DNA replication.  |  Kaufman, PD., et al. 1995. Cell. 81: 1105-14. PMID: 7600578
12. Nucleosome assembly by a complex of CAF-1 and acetylated histones H3/H4.  |  Verreault, A., et al. 1996. Cell. 87: 95-104. PMID: 8858152

The preferred HP1 antibody is HP1 Antibody (E-6): sc-515341.