HoxC5 CRISPR/Cas9 KO Plasmid (h): sc-406548
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HoxC5 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HoxC5 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HoxC5 Antibody (1E10): sc-517171
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HoxC5 CRISPR/Cas9 KO Plasmid (h) | sc-406548 | 20 µg | $397.00 |
Overview
HOXC5 encodes the transcription factor HoxC5, a homeobox DNA-binding protein that contributes to anterior–posterior patterning and regional identity during embryonic development. In differentiated tissues, HoxC5 can influence programs of cell fate specification, morphogenesis, and migration by regulating transcriptional networks that interface with chromatin organization and developmental signaling pathways. Dysregulated HOX gene expression patterns, including HOXC5, have been reported in multiple tumor types and are frequently associated with altered differentiation states, invasiveness, and transcriptional rewiring. As a developmental regulator, HOXC5 is also relevant for studying lineage commitment, epithelial–mesenchymal plasticity, and context-dependent gene regulatory circuitry.
HoxC5 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the HOXC5 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the HOXC5 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the HOXC5 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HoxC5 protein expression.
This CRISPR knockout system enables efficient generation of HOXC5-deficient cell models for investigation of HoxC5 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting HOXC5 exon(s) critical for HoxC5 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple HOXC5 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HoxC5 CRISPR/Cas9 KO Plasmid (h) and HoxC5 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the HOXC5 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HoxC5 HDR Plasmid (h) and HoxC5 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by HOXC5 homology arms to support homology-directed repair at defined HOXC5 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.