HoxB4 CRISPR/Cas9 KO Plasmid (h): sc-404013
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HoxB4 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HoxB4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HoxB4 Antibody (D-1): sc-365927
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HoxB4 CRISPR/Cas9 KO Plasmid (h) | sc-404013 | 20 µg | $397.00 |
Overview
HOXB4 encodes the homeobox transcription factor HoxB4, a sequence-specific DNA-binding regulator that helps establish anterior–posterior patterning during development and controls lineage specification programs in hematopoietic and other progenitor compartments. HoxB4 activity coordinates transcriptional networks governing stem/progenitor self-renewal, differentiation timing, and cell-cycle progression, integrating with broader developmental signaling and chromatin regulatory pathways. Dysregulated HOXB4 expression has been reported in hematologic malignancy contexts and is frequently studied as part of HOX-cluster regulatory circuitry, enhancer–promoter architecture, and epigenetic control of cell identity.
HoxB4 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the HOXB4 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the HOXB4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the HOXB4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HoxB4 protein expression.
This CRISPR knockout system enables efficient generation of HOXB4-deficient cell models for investigation of HoxB4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting HOXB4 exon(s) critical for HoxB4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple HOXB4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HoxB4 CRISPR/Cas9 KO Plasmid (h) and HoxB4 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the HOXB4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HoxB4 HDR Plasmid (h) and HoxB4 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by HOXB4 homology arms to support homology-directed repair at defined HOXB4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.