HoxB4 Antibody (D-1): sc-365927
- HoxB4 Antibody (D-1) is a mouse monoclonal IgG1 κ HoxB4 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 91-140 mapping within an internal region of HoxB4 of human origin
- HoxB4 Antibody (D-1) is recommended for detection of HoxB4 of human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-HoxB4 Antibody (D-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for HoxB4 Antibody (D-1) for WB and IHC(P) applications. These reagents are now offered in bundles with HoxB4 Antibody (D-1) (see ordering information below).
HoxB4 Antibody (D-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects HoxB4 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-HoxB4 antibody (D-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. HoxB4 protein, encoded by the HOXB4 gene, is a nuclear phosphoprotein that plays a crucial role in regulating hematopoietic stem cell dynamics, balancing self-renewal and differentiation. This regulation is vital for maintaining proper blood cell development and tissue homeostasis. HoxB4′s function is influenced by interactions with transcription factors such as USF-1 and USF-2, which are modulated by cytokines that promote stem cell self-renewal. Additionally, HoxB4 depends on AP-1 expression to drive changes in cellular proliferation and differentiation, which elevates cyclin D1 levels, linking HoxB4 to essential components of the cell cycle machinery. HoxB4 is involved in down-regulating c-Myc expression, highlighting HoxB4′s multifaceted role in cellular regulation. HoxB4 is expressed in developing hair follicles and in cell lines such as K-562 and HL-60, making HoxB4 a significant marker for studies related to hematopoiesis and stem cell biology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
HoxB4 Antibody (D-1) References:
- c-myc intron element-binding proteins are required for 1, 25-dihydroxyvitamin D3 regulation of c-myc during HL-60 cell differentiation and the involvement of HOXB4. | Pan, Q. and Simpson, RU. 1999. J Biol Chem. 274: 8437-44. PMID: 10085075
- AP-1 complex is effector of Hox-induced cellular proliferation and transformation. | Krosl, J. and Sauvageau, G. 2000. Oncogene. 19: 5134-41. PMID: 11064450
- Hematopoietic expression of HOXB4 is regulated in normal and leukemic stem cells through transcriptional activation of the HOXB4 promoter by upstream stimulating factor (USF)-1 and USF-2. | Giannola, DM., et al. 2000. J Exp Med. 192: 1479-90. PMID: 11085749
- Hoxa4 expression in developing mouse hair follicles and skin. | Packer, AI., et al. 2000. Mech Dev. 99: 153-7. PMID: 11091084
- Antisense knockout of HOXB4 blocks 1,25-dihydroxyvitamin D3 inhibition of c-myc expression. | Pan, Q. and Simpson, RU. 2001. J Endocrinol. 169: 153-9. PMID: 11250656
- Two homoeo box loci mapped in evolutionarily related mouse and human chromosomes. | Rabin, M., et al. Nature. 314: 175-8. PMID: 4038785
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HoxB4 Antibody (D-1) | sc-365927 | 200 µg/ml | $322.00 | |||
HoxB4 Antibody (D-1): m-IgG Fc BP-HRP Bundle | sc-529515 | 200 µg Ab; 10 µg BP | $361.00 | |||
HoxB4 Antibody (D-1): m-IgGκ BP-HRP Bundle | sc-522426 | 200 µg Ab, 40 µg BP | $361.00 | |||
HoxB4 Antibody (D-1): m-IgG1 BP-HRP Bundle | sc-543554 | 200 µg Ab; 20 µg BP | $361.00 | |||
HoxB4 Antibody (D-1) AC | sc-365927 AC | 500 µg/ml, 25% agarose | $424.00 | |||
HoxB4 Antibody (D-1) HRP | sc-365927 HRP | 200 µg/ml | $322.00 | |||
HoxB4 Antibody (D-1) FITC | sc-365927 FITC | 200 µg/ml | $336.00 | |||
HoxB4 Antibody (D-1) PE | sc-365927 PE | 200 µg/ml | $349.00 | |||
HoxB4 Antibody (D-1) Alexa Fluor® 488 | sc-365927 AF488 | 200 µg/ml | $364.00 | |||
HoxB4 Antibody (D-1) Alexa Fluor® 546 | sc-365927 AF546 | 200 µg/ml | $364.00 | |||
HoxB4 Antibody (D-1) Alexa Fluor® 594 | sc-365927 AF594 | 200 µg/ml | $364.00 | |||
HoxB4 Antibody (D-1) Alexa Fluor® 647 | sc-365927 AF647 | 200 µg/ml | $364.00 | |||
HoxB4 Antibody (D-1) Alexa Fluor® 680 | sc-365927 AF680 | 200 µg/ml | $364.00 | |||
HoxB4 Antibody (D-1) Alexa Fluor® 790 | sc-365927 AF790 | 200 µg/ml | $364.00 |